Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells. S D. Thorpe C T. Buckley T Vinardell Fergal J. O'Brien V A. Campbell Daniel J. Kelly 10779/rcsi.10764686.v1 https://repository.rcsi.com/articles/journal_contribution/Dynamic_compression_can_inhibit_chondrogenesis_of_mesenchymal_stem_cells_/10764686 <p>The objective of this study was to investigate the influence of dynamic compressive loading on chondrogenesis of mesenchymal stem cells (MSCs) in the presence of TGF-beta3. Isolated porcine MSCs were suspended in 2% agarose and subjected to intermittent dynamic compression (10% strain) for a period of 42 days in a dynamic compression bioreactor. After 42 days in culture, the free-swelling specimens exhibited more intense alcian blue staining for proteoglycans, while immunohistochemical analysis revealed increased collagen type II immunoreactivity. Glycosaminoglycan (GAG) content increased with time for both free-swelling and dynamically loaded constructs, and by day 42 it was significantly higher in both the core (2.5+/-0.21%w/w vs. 0.94+/-0.03%w/w) and annulus (1.09+/-0.09%w/w vs. 0.59+/-0.08%w/w) of free-swelling constructs compared to dynamically loaded constructs. This result suggests that further optimization is required in controlling the biomechanical and/or the biochemical environment if such stimuli are to have beneficial effects in generating functional cartilaginous tissue.</p> 2019-11-22 15:04:43 Animals Bioreactors Cell Culture Techniques Chondrogenesis Collagen Type II Compressive Strength Glycosaminoglycans Mesenchymal Stem Cells Swine Transforming Growth Factor beta3 Anatomy