Development and characterisation of a collagen nano-hydroxyapatite composite scaffold for bone tissue engineering. Grainne M. Cunniffe Glenn R. Dickson Sonia Partap Kenneth T. Stanton Fergal J. O'Brien 10779/rcsi.10765679.v1 https://repository.rcsi.com/articles/journal_contribution/Development_and_characterisation_of_a_collagen_nano-hydroxyapatite_composite_scaffold_for_bone_tissue_engineering_/10765679 <p>Bone regeneration requires scaffolds that possess suitable mechanical and biological properties. This study sought to develop a novel collagen-nHA biocomposite scaffold via two new methods. Firstly a stable nHA suspension was produced and added to a collagen slurry (suspension method), and secondly, porous collagen scaffolds were immersed in nHA suspension after freeze-drying (immersion method). Significantly stronger constructs were produced using both methods compared to collagen only scaffolds, with a high porosity maintained (>98.9%). It was found that Coll-nHA composite scaffolds produced by the suspension method were up to 18 times stiffer than the collagen control (5.50 +/- 1.70 kPa vs. 0.30 +/- 0.09 kPa). The suspension method was also more reproducible, and the quantity of nHA incorporated could be varied with greater ease than with the immersion technique. In addition, Coll-nHA composites display excellent biological activity, demonstrating their potential as bone graft substitutes in orthopaedic regenerative medicine.</p> 2019-11-22 15:08:52 Animals Biocompatible Materials Bone Regeneration Bone Substitutes Bone and Bones Cattle Cell Proliferation Cells Cultured Collagen Durapatite Guided Tissue Regeneration Mice Spectroscopy Fourier Transform Infrared Tissue Engineering Tissue Scaffolds Anatomy