Development and characterisation of a collagen nano-hydroxyapatite composite scaffold for bone tissue engineering.
Grainne M. Cunniffe
Glenn R. Dickson
Sonia Partap
Kenneth T. Stanton
Fergal J. O'Brien
10779/rcsi.10765679.v1
https://repository.rcsi.com/articles/journal_contribution/Development_and_characterisation_of_a_collagen_nano-hydroxyapatite_composite_scaffold_for_bone_tissue_engineering_/10765679
<p>Bone regeneration requires scaffolds that possess suitable mechanical and biological properties. This study sought to develop a novel collagen-nHA biocomposite scaffold via two new methods. Firstly a stable nHA suspension was produced and added to a collagen slurry (suspension method), and secondly, porous collagen scaffolds were immersed in nHA suspension after freeze-drying (immersion method). Significantly stronger constructs were produced using both methods compared to collagen only scaffolds, with a high porosity maintained (>98.9%). It was found that Coll-nHA composite scaffolds produced by the suspension method were up to 18 times stiffer than the collagen control (5.50 +/- 1.70 kPa vs. 0.30 +/- 0.09 kPa). The suspension method was also more reproducible, and the quantity of nHA incorporated could be varied with greater ease than with the immersion technique. In addition, Coll-nHA composites display excellent biological activity, demonstrating their potential as bone graft substitutes in orthopaedic regenerative medicine.</p>
2019-11-22 15:08:52
Animals
Biocompatible Materials
Bone Regeneration
Bone Substitutes
Bone and Bones
Cattle
Cell Proliferation
Cells
Cultured
Collagen
Durapatite
Guided Tissue Regeneration
Mice
Spectroscopy
Fourier Transform Infrared
Tissue Engineering
Tissue Scaffolds
Anatomy