10779/rcsi.10766306.v1 Laura Corrigan Laura Corrigan Caroline Jefferies Caroline Jefferies T Clive Lee T Clive Lee Jacqueline Daly Jacqueline Daly Evaluation and optimization of IgY spin column technology in the depletion of abundant proteins from human serum. Royal College of Surgeons in Ireland 2019 Biological Markers Blood Proteins Chromatography Liquid Electrophoresis Gel Two-Dimensional Polyacrylamide Gel Humans Immunoglobulins Proteomics Anatomy 2019-11-22 15:11:30 Journal contribution https://repository.rcsi.com/articles/journal_contribution/Evaluation_and_optimization_of_IgY_spin_column_technology_in_the_depletion_of_abundant_proteins_from_human_serum_/10766306 <p>Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin columns. However, modification of the manufacturer's protocol, subjecting samples to two rounds of depletion, improved the number of proteins resolved by 2-DE. With alteration of the manufacturer protocol, the Seppro(®) IgY14 spin column can produce depleted serum with an increased number of spots resolved by 2-DE compared to untreated serum.</p>