10779/rcsi.10766306.v1
Laura Corrigan
Laura
Corrigan
Caroline Jefferies
Caroline
Jefferies
T Clive Lee
T Clive
Lee
Jacqueline Daly
Jacqueline
Daly
Evaluation and optimization of IgY spin column technology in the depletion of abundant proteins from human serum.
Royal College of Surgeons in Ireland
2019
Biological Markers
Blood Proteins
Chromatography
Liquid
Electrophoresis
Gel
Two-Dimensional
Polyacrylamide Gel
Humans
Immunoglobulins
Proteomics
Anatomy
2019-11-22 15:11:30
Journal contribution
https://repository.rcsi.com/articles/journal_contribution/Evaluation_and_optimization_of_IgY_spin_column_technology_in_the_depletion_of_abundant_proteins_from_human_serum_/10766306
<p>Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin columns. However, modification of the manufacturer's protocol, subjecting samples to two rounds of depletion, improved the number of proteins resolved by 2-DE. With alteration of the manufacturer protocol, the Seppro(®) IgY14 spin column can produce depleted serum with an increased number of spots resolved by 2-DE compared to untreated serum.</p>