10779/rcsi.10768865.v1
Liam Burke
Liam
Burke
Hilary Humphreys
Hilary
Humphreys
Deirdre Fitzgerald-Hughes
Deirdre
Fitzgerald-Hughes
The revolving door between hospital and community: extended-spectrum beta-lactamase-producing Escherichia coli in Dublin.
Royal College of Surgeons in Ireland
2019
Adolescent
Adult
Aged
80 and over
Anti-Bacterial Agents
Child
Community-Acquired Infections
Cross Infection
Drug Resistance
Bacterial
Electrophoresis
Gel
Pulsed-Field
Escherichia coli
Escherichia coli Infections
Female
Humans
Ireland
Male
Microbial Sensitivity Tests
Middle Aged
Phylogeny
Young Adult
beta-Lactamases
Clinical Microbiology
2019-11-22 15:22:18
Journal contribution
https://repository.rcsi.com/articles/journal_contribution/The_revolving_door_between_hospital_and_community_extended-spectrum_beta-lactamase-producing_Escherichia_coli_in_Dublin_/10768865
<p>BACKGROUND: Escherichia coli that produce extended-spectrum beta-lactamases (ESBLs) are an increasing cause of healthcare-associated infection, and community healthcare facilities may be a reservoir for important epidemic clones.</p>
<p>AIM: To characterize retrospectively and investigate the epidemiology of ESBL-producing E. coli collected in a Dublin hospital, during 2009 and 2010, and to investigate the dissemination of specific clones within hospital and community healthcare facilities.</p>
<p>METHODS: Pulsed-field gel electrophoresis (PFGE) was used to determine the genetic relatedness of 100 ESBL-producing E. coli isolates. Phylogenetic groups were determined and the O25b-ST131 clone identified in the collection. The genetic data were correlated with antimicrobial susceptibility, clinical and demographic data to explore the epidemiology of specific clones.</p>
<p>FINDINGS: Phylogenetic groups B2 (62%) and D (18%) were the most common and were associated with non-urinary isolates (P</p>
<p>CONCLUSIONS: E. coli O25b-ST131 is largely responsible for ESBL-producing E. coli in LTCFs in Dublin. The distribution of ESBL-producing E. coli in our hospital and community highlights a 'revolving door' through which these resistant bacteria spread and disseminate.</p>