10779/rcsi.10768865.v1 Liam Burke Liam Burke Hilary Humphreys Hilary Humphreys Deirdre Fitzgerald-Hughes Deirdre Fitzgerald-Hughes The revolving door between hospital and community: extended-spectrum beta-lactamase-producing Escherichia coli in Dublin. Royal College of Surgeons in Ireland 2019 Adolescent Adult Aged 80 and over Anti-Bacterial Agents Child Community-Acquired Infections Cross Infection Drug Resistance Bacterial Electrophoresis Gel Pulsed-Field Escherichia coli Escherichia coli Infections Female Humans Ireland Male Microbial Sensitivity Tests Middle Aged Phylogeny Young Adult beta-Lactamases Clinical Microbiology 2019-11-22 15:22:18 Journal contribution https://repository.rcsi.com/articles/journal_contribution/The_revolving_door_between_hospital_and_community_extended-spectrum_beta-lactamase-producing_Escherichia_coli_in_Dublin_/10768865 <p>BACKGROUND: Escherichia coli that produce extended-spectrum beta-lactamases (ESBLs) are an increasing cause of healthcare-associated infection, and community healthcare facilities may be a reservoir for important epidemic clones.</p> <p>AIM: To characterize retrospectively and investigate the epidemiology of ESBL-producing E. coli collected in a Dublin hospital, during 2009 and 2010, and to investigate the dissemination of specific clones within hospital and community healthcare facilities.</p> <p>METHODS: Pulsed-field gel electrophoresis (PFGE) was used to determine the genetic relatedness of 100 ESBL-producing E. coli isolates. Phylogenetic groups were determined and the O25b-ST131 clone identified in the collection. The genetic data were correlated with antimicrobial susceptibility, clinical and demographic data to explore the epidemiology of specific clones.</p> <p>FINDINGS: Phylogenetic groups B2 (62%) and D (18%) were the most common and were associated with non-urinary isolates (P</p> <p>CONCLUSIONS: E. coli O25b-ST131 is largely responsible for ESBL-producing E. coli in LTCFs in Dublin. The distribution of ESBL-producing E. coli in our hospital and community highlights a 'revolving door' through which these resistant bacteria spread and disseminate.</p>