Burke, Liam Humphreys, Hilary Fitzgerald-Hughes, Deirdre The revolving door between hospital and community: extended-spectrum beta-lactamase-producing Escherichia coli in Dublin. <p>BACKGROUND: Escherichia coli that produce extended-spectrum beta-lactamases (ESBLs) are an increasing cause of healthcare-associated infection, and community healthcare facilities may be a reservoir for important epidemic clones.</p> <p>AIM: To characterize retrospectively and investigate the epidemiology of ESBL-producing E. coli collected in a Dublin hospital, during 2009 and 2010, and to investigate the dissemination of specific clones within hospital and community healthcare facilities.</p> <p>METHODS: Pulsed-field gel electrophoresis (PFGE) was used to determine the genetic relatedness of 100 ESBL-producing E. coli isolates. Phylogenetic groups were determined and the O25b-ST131 clone identified in the collection. The genetic data were correlated with antimicrobial susceptibility, clinical and demographic data to explore the epidemiology of specific clones.</p> <p>FINDINGS: Phylogenetic groups B2 (62%) and D (18%) were the most common and were associated with non-urinary isolates (P</p> <p>CONCLUSIONS: E. coli O25b-ST131 is largely responsible for ESBL-producing E. coli in LTCFs in Dublin. The distribution of ESBL-producing E. coli in our hospital and community highlights a 'revolving door' through which these resistant bacteria spread and disseminate.</p> Adolescent;Adult;Aged;80 and over;Anti-Bacterial Agents;Child;Community-Acquired Infections;Cross Infection;Drug Resistance;Bacterial;Electrophoresis;Gel;Pulsed-Field;Escherichia coli;Escherichia coli Infections;Female;Humans;Ireland;Male;Microbial Sensitivity Tests;Middle Aged;Phylogeny;Young Adult;beta-Lactamases;Clinical Microbiology 2019-11-22
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