Harney, Donal F. Butler, Ryan K. Edwards, Richard J. Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics approach. <p>BACKGROUND: Previously it has been shown that insulin-mediated tyrosine phosphorylation of myosin heavy chain is concomitant with enhanced association of C-terminal SRC kinase during skeletal muscle differentiation. We sought to identify putative site(s) for this phosphorylation event.</p> <p>RESULTS: A combined bioinformatics approach of motif prediction and evolutionary and structural analyses identified tyrosines163 and 1856 of the skeletal muscle heavy chain as the leading candidate for the sites of insulin-mediated tyrosine phosphorylation.</p> <p>CONCLUSION: Our work is suggestive that tyrosine phosphorylation of myosin heavy chain, whether in skeletal muscle or in platelets, is a significant event that may initiate cytoskeletal reorganization of muscle cells and platelets. Our studies provide a good starting point for further functional analysis of MHC phosphor-signalling events within different cells.</p> Algorithms;Amino Acid Sequence;Animals;Cell Differentiation;Chickens;Computational Biology;Humans;Molecular Sequence Data;Muscle;Skeletal;Myosin Heavy Chains;Phosphorylation;Phosphotyrosine;Phylogeny;Sequence Homology;Amino Acid;Clinical Pharmacology and Therapeutics 2019-11-22
    https://repository.rcsi.com/articles/journal_contribution/Tyrosine_phosphorylation_of_myosin_heavy_chain_during_skeletal_muscle_differentiation_an_integrated_bioinformatics_approach_/10783148