%0 Journal Article %A Vencken, Sebastian %A Hassan, Tidi %A McElvaney, Noel G. %A Smith, Stephen GJ %A Greene, Catherine M. %D 2019 %T miR-CATCH: microRNA capture affinity technology. %U https://repository.rcsi.com/articles/journal_contribution/miR-CATCH_microRNA_capture_affinity_technology_/10785416 %2 https://repository.rcsi.com/ndownloader/files/19298450 %K Base Pairing %K Base Sequence %K Cell Line %K Humans %K MicroRNAs %K Molecular Sequence Data %K Nucleic Acid Hybridization %K Oligonucleotides %K Antisense %K RNA %K Messenger %K Medicine %X

Several experimental methods exist to explore the microRNA (miRNA) regulome. These methods almost exclusively focus on multiple targets bound to a single, or perhaps a few miRNAs of interest. Here, we describe a microRNA capture affinity technology (miR-CATCH) which uses an affinity capture oligonucleotide to co-purify a single target messenger RNA (mRNA) together with all its endogenously bound miRNAs. This bench-top method is similar to RNA immunoprecipitation (RIP) and provides an experimental alternative to computational miRNA target prediction.

%I Royal College of Surgeons in Ireland