Biofilm and the role of the ica operon and aap in Staphylococcus epidermidis isolates causing neurosurgical meningitis.
Niall T. Stevens
M Tharmabala
T Dillane
Catherine M. Greene
James P. O'Gara
Hilary Humphreys
10779/rcsi.10785752.v1
https://repository.rcsi.com/articles/journal_contribution/Biofilm_and_the_role_of_the_ica_operon_and_aap_in_Staphylococcus_epidermidis_isolates_causing_neurosurgical_meningitis_/10785752
Fifty-five Staphylococcus epidermidis isolates, classified as contaminants or causing device-related meningitis, from external ventricular drain (EVD) and non-EVD cerebrospinal fluid specimens were characterized. Thirty-three of 42 (78.6%) meningitis isolates were PCR-positive for ica and aap, known determinants of polysaccharide- and protein-mediated biofilm production, whereas five of 13 (38.5%) contaminants were ica- and aap-negative; 71.4% of meningitis isolates and 84.6% of contaminants produced biofilm. ica+aap+ meningitis isolates produced more biofilm than ica+aap- isolates (p 0.0020). ica+aap- isolates did not produce more biofilm than ica-aap+ isolates (p 0.4368). Apparently, ica and aap are associated with biofilm production in S. epidermidis device-related meningitis isolates.
2019-11-22 16:36:14
Bacterial Adhesion
Bacterial Typing Techniques
Biofilms
Cerebrospinal Fluid Shunts
DNA
Bacterial
Equipment Contamination
Genes
Genotype
Humans
Meningitis
Operon
Polymerase Chain Reaction
Prosthesis-Related Infections
Staphylococcal Infections
Staphylococcus epidermidis
Medicine