%0 Journal Article %A Murphy, Niamh %A Yamamoto, Akitaka %A Henshall, David C. %D 2019 %T Detection of 14-3-3zeta in cerebrospinal fluid following experimentally evoked seizures. %U https://repository.rcsi.com/articles/journal_contribution/Detection_of_14-3-3zeta_in_cerebrospinal_fluid_following_experimentally_evoked_seizures_/10790195 %2 https://repository.rcsi.com/ndownloader/files/19302665 %K 14-3-3 Proteins %K Animals %K Biological Markers %K Hippocampus %K Kainic Acid %K Male %K Nerve Tissue Proteins %K Rats %K Sprague-Dawley %K Seizures %K Physiology %K Medical Physics %X

Surrogate and peripheral (bio)markers of neuronal injury may be of value in assessing effects of seizures on the brain or epilepsy development following trauma. The presence of 14-3-3 isoforms in cerebrospinal fluid (CSF) is a diagnostic indicator of Creutzfeldt-Jakob disease but these proteins may also be present following acute neurological insults. Here, we examined neuronal and 14-3-3 proteins in CSF from rats after seizures. Seizures induced by intra-amygdala microinjection of 0.1 microg kainic acid (KA) caused damage which was mainly restricted to the ipsilateral CA3 subfield of the hippocampus. 14-3-3zeta was detected at significant levels in CSF sampled 4 h after seizures compared with near absence in control CSF. Neuron-specific nuclear protein (NeuN) was also elevated in CSF in seizure rats. CSF 14-3-3zeta levels were significantly lower in rats treated with 0.01 microg KA. These data suggest the presence of 14-3-3zeta within CSF may be a biomarker of acute seizure damage.

%I Royal College of Surgeons in Ireland