Bacon, Siobhán Engelbrecht, Britta Schmid, Jasmin Pfeiffer, Shona Gallagher, Ross McCarthy, Ailbhe Burke, Marie Concannon, Caoimhín Prehn, Jochen HM Byrne, Maria M. MicroRNA-224 is Readily Detectable in Urine of Individuals with Diabetes Mellitus and is a Potential Indicator of Beta-Cell Demise. <p>MicroRNA (miRNA) are a class of non-coding, 19-25 nucleotide RNA critical for network-level regulation of gene expression. miRNA serve as paracrine signaling molecules. Using an unbiased array approach, we previously identified elevated levels of miR-224 and miR-103 to be associated with a monogenic form of diabetes; HNF1A-MODY. miR-224 is a novel miRNA in the field of diabetes. We sought to explore the role of miR-224 as a potential biomarker in diabetes, and whether such diabetes-associated-miRNA can also be detected in the urine of patients. Absolute levels of miR-224 and miR-103 were determined in the urine of n = 144 individuals including carriers of a HNF1A mutation, participants with type 1 diabetes mellitus (T1DM), type 2 diabetes mellitus (T2DM) and normal controls. Expression levels were correlated with clinical and biochemical parameters. miR-224 was significantly elevated in the urine of carriers of a HNF1A mutation and participants with T1DM. miR-103 was highly expressed in urine across all diabetes cohorts when compared to controls. For both miR-224 and-103, we found a significant correlation between serum and urine levels (p < 0.01). We demonstrate that miRNA can be readily detected in the urine independent of clinical indices of renal dysfunction. We surmise that the differential expression levels of miR-224 in both HNF1A-MODY mutation carriers and T1DM may be an attempt to compensate for beta-cell demise.</p> Diabetes Associated IcroRNA;Insulin Secretion; miR-103; miR-224; Urinary Biomarker;Physiology;Medical Physics 2019-11-22
    https://repository.rcsi.com/articles/journal_contribution/MicroRNA-224_is_Readily_Detectable_in_Urine_of_Individuals_with_Diabetes_Mellitus_and_is_a_Potential_Indicator_of_Beta-Cell_Demise_/10793501