10779/rcsi.10794173.v1
Klaus O. Schubert
Klaus O.
Schubert
Melanie Föcking
Melanie
Föcking
David R. Cotter
David R.
Cotter
Proteomic pathway analysis of the hippocampus in schizophrenia and bipolar affective disorder implicates 14-3-3 signaling, aryl hydrocarbon receptor signaling, and glucose metabolism: potential roles in GABAergic interneuron pathology.
Royal College of Surgeons in Ireland
2019
14-3-3 Proteins
Adult
Bipolar Disorder
Female
Glucose
Hippocampus
Humans
Interneurons
Male
Middle Aged
Protein Interaction Maps
Proteomics
Receptors
Aryl Hydrocarbon
Schizophrenia
Signal Transduction
Young Adult
gamma-Aminobutyric Acid
Psychiatry (incl. Psychotherapy)
2019-11-22 17:08:17
Journal contribution
https://repository.rcsi.com/articles/journal_contribution/Proteomic_pathway_analysis_of_the_hippocampus_in_schizophrenia_and_bipolar_affective_disorder_implicates_14-3-3_signaling_aryl_hydrocarbon_receptor_signaling_and_glucose_metabolism_potential_roles_in_GABAergic_interneuron_pathology_/10794173
<p>Neuropathological changes of the hippocampus have been associated with psychotic disorders such as schizophrenia and bipolar disorder. Recent work has particularly implicated hippocampal GABAergic interneurons in the pathophysiology of these diseases. However, the molecular mechanisms underlying structural and cellular hippocampal pathology remain poorly understood. We used data from comprehensive difference-in-gel electrophoresis (2-D DIGE) investigations of postmortem human hippocampus of people with schizophrenia and bipolar disorder, covering the acidic (isoelectric point (pI) between pH4 and 7) and, separately, the basic (pI between pH6 and 11) sub-proteome, for Ingenuity Pathway Analysis (IPA) of implicated protein networks and pathways. Comparing disease and control cases, we identified 58 unique differentially expressed proteins in schizophrenia, and 70 differentially expressed proteins in bipolar disorder, using mass spectrometry. IPA implicated, most prominently, 14-3-3 and aryl hydrocarbon receptor signaling in schizophrenia, and gluconeogenesis/glycolysis in bipolar disorder. Both disorders were characterized by alterations of proteins involved in the oxidative stress response, mitochondrial function, and protein-endocytosis, -trafficking, -degradation, and -ubiquitination. These findings are interpreted with a focus on GABAergic interneuron pathology in the hippocampus.</p>