10779/rcsi.10991486.v1 Aoife M. McKeon Aoife M. McKeon Alan Egan Alan Egan Jay Chandanshive Jay Chandanshive Helena McMahon Helena McMahon Darren M. Griffith Darren M. Griffith Novel Improved Synthesis of HSP70 Inhibitor, Pifithrin-μ. In Vitro Synergy Quantification of Pifithrin-μ Combined with Pt Drugs in Prostate and Colorectal Cancer Cells. Royal College of Surgeons in Ireland 2019 Chou-Talalay method cancer combination study heat shock protein 70 pifithrin-μ platinum synthesis. 2019-11-23 11:50:48 Journal contribution https://repository.rcsi.com/articles/journal_contribution/Novel_Improved_Synthesis_of_HSP70_Inhibitor_Pifithrin-_In_Vitro_Synergy_Quantification_of_Pifithrin-_Combined_with_Pt_Drugs_in_Prostate_and_Colorectal_Cancer_Cells_/10991486 <p>We describe a novel improved approach to the synthesis of the important and well-known heat shock protein 70 inhibitor (HSP70), pifithrin-μ, with corresponding and previously unreported characterisation. The first example of a combination study comprising HSP70 inhibitor pifithrin-μ and cisplatin or oxaliplatin is reported. We have determined, using the Chou-Talalay method, (i) moderate synergistic and synergistic effects in co-treating PC-3 prostate cancer cells with pifithrin-μ and cisplatin and (ii) significant synergistic effects including strong synergism in cotreating HT29 colorectal cancer cells with oxaliplatin and pifithrin-μ.</p>