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Development of collagen-hydroxyapatite scaffolds incorporating PL.pdf (1.19 MB)

Development of collagen-hydroxyapatite scaffolds incorporating PLGA and alginate microparticles for the controlled delivery of rhBMP-2 for bone tissue engineering.

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Version 1 2019-11-22, 15:12
journal contribution
posted on 2019-11-22, 15:12 authored by Elaine Quinlan, Adolfo López-Noriega, Emmet Thompson, Helena M. Kelly, Sally-Ann Cryan, Fergal O'Brien

The spatiotemporally controlled delivery of the pro-osteogenic factor rhBMP-2 would overcome most of the severe secondary effects linked to the products delivering this protein for bone regeneration. With this in mind, the aim of the present work was to develop a controlled rhBMP-2 release system using collagen-hydroxyapatite (CHA) scaffolds, which had been previously optimized for bone regeneration, as delivery platforms to produce a device with enhanced capacity for bone repair. Spray-drying and emulsion techniques were used to encapsulate bioactive rhBMP-2 in alginate and PLGA microparticles, with a high encapsulation efficiency. After incorporation of these microparticles into the scaffolds, rhBMP-2 was delivered in a sustained fashion for up to 28days. When tested in vitro with osteoblasts, these eluting materials showed an enhanced pro-osteogenic effect. From these results, an optimal rhBMP-2 eluting scaffold composition was selected and implanted in critical-sized calvarial defects in a rat model, where it demonstrated an excellent healing capacity in vivo. These platforms have an immense potential in the field of tissue regeneration; by tuning the specific therapeutic molecule to the tissue of interest and by utilizing different collagen-based scaffolds, similar systems can be developed for enhancing the healing of a diverse range of tissues and organs.

Funding

Science Foundation Ireland. Enterprise Ireland. European Research Council

History

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The original article is available at www.sciencedirect.com

Published Citation

Quinlan E, López-Noriega A, Thompson E, Kelly HM, Cryan SA, O'Brien FJ. Development of collagen-hydroxyapatite scaffolds incorporating PLGA and alginate microparticles for the controlled delivery of rhBMP-2 for bone tissue engineering. Journal of Controled Release. 2015;198:71-9.

Publication Date

2015-01-28

Publisher

Elsevier Science Publishers

PubMed ID

25481441

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