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Incorporation of PLGA nanoparticles into porous chitosan-gelatin.pdf (3.33 MB)

Incorporation of PLGA nanoparticles into porous chitosan-gelatin scaffolds: influence on the physical properties and cell behavior.

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Version 2 2021-12-10, 09:23
Version 1 2019-11-22, 17:21
journal contribution
posted on 2021-12-10, 09:23 authored by Vijay Kumar Nandagiri, Piergiorgio Gentile, Valeria Chiono, Chiara Tonda-Turo, Amos Matsiko, Zeibun Ramtoola, Franco Maria Montevecchi, Gianluca Ciardelli, Zebunissa Ramtoola

Bone regeneration can be accelerated by localized delivery of appropriate growth factors/biomolecules. Localized delivery can be achieved by a 2-level system: (i) incorporation of biomolecules within biodegradable particulate carriers (nanoparticles), and (ii) inclusion of such particulate carriers (nanoparticles) into suitable porous scaffolds. In this study, freeze-dried porous chitosan-gelatin scaffolds (CH-G: 1:2 ratio by weight) were embedded with various amounts of poly(lactide-co-glycolide) (PLGA) nanoparticles, precisely 16.6%, 33.3% and 66.6% (respect to CH-G weight). Scaffolds loaded with PLGA nanoparticles were subjected to physico-mechanical and biological characterizations including morphological analysis, swelling and dissolution tests, mechanical compression tests and cell viability tests. Results showed that incorporation of PLGA nanoparticles into porous crosslinked CH-G scaffolds: (i) changed the micro-architecture of the scaffolds in terms of mean pore diameter and pore size distribution, (ii) reduced the dissolution degree of the scaffolds, and (iii) increased the compressive modulus. On the other hand, the water uptake behavior of CH-G scaffolds containing PLGA nanoparticles significantly decreased. The incorporation of PLGA nanoparticles did not affect the biocompatibility of CH-G scaffolds.

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This article is also available at http://www.sciencedirect.com/science/article/pii/S1751616111001020

Published Citation

Nandagirl VK, Gentile P, Chiono V, Tonda-Turo C, Matsiko A, Ramtoola Z, Montevecchi FM, Ciardelli G. Incorporation of PLGA nanoparticles into porous chitosan-gelatin scaffolds: influence on the physical properties and cell behavior. Journal of the Mechanical Behavior of Biomedical Materials. 2011;4(7):1318-27.

Publication Date

2011-01-01

PubMed ID

21783141

Department/Unit

  • Anatomy and Regenerative Medicine
  • School of Pharmacy and Biomolecular Sciences

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