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Adolescent Risperidone treatment alters protein expression associated with protein trafficking and cellular metabolism in the adult rat prefrontal cortex.

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posted on 22.11.2019 by Lorna Farrelly, Patrick Dicker, Kieran Wynne, Jane A. English, Gerard Cagney, Melanie Föcking, David R. Cotter

The prefrontal cortex (PFC) is associated with mental health illnesses including schizophrenia, depression, bipolar disorder, and autism spectrum disorders. It richly expresses neuroreceptors which are the target for antipsychotics. However, as the precise mechanism of action of antipsychotic medications is not known, proteomic studies of the effects of antipsychotic drugs on the brain are warranted. In the current study, we aimed to characterize protein expression in the adult rodent PFC (n = 5 per group) following low-dose treatment with Risperidone or saline in adolescence (postnatal days 34-47). The PFC was examined by triplicate 1 h runs of label-free LC-MS/MS. The raw mass spectral data were analyzed with the MaxQuant(TM) software. Statistical analysis was carried out using SAS® Version 9.1. Pathway and functional analysis was performed with IngenuityPathway Analysis and in the Database for Annotation, Visualization and Integrated Discovery (DAVID), respectively, the most implicated pathways were found to be related to mitochondrial function, protein trafficking, and the cytoskeleton. This report adds to the current repertoire of data available concerning the effects of antipsychotic drugs on the brain and sheds light on their biological mechanisms. The MS data have been deposited with the ProteomeXchange Consortium with dataset identifier PXD000480.

Funding

Animal tissue was provided by Professor Ina Weiner in the School of Psychological Sciences in the University of Tel Aviv. Wethank the staff of the Mass Spectrometry Core Unit in the Conway Institute in the University College of Dublin for technical assistance. This work was supported by Science Foundation Ireland (RFP1304 to D.R.C.).

History

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This is the pre-peer reviewed version of the following article: Farrelly LA, Dicker P, Wynne K, English J, Cagney G, Föcking M, Cotter DR. Adolescent Risperidone treatment alters protein expression associated with protein trafficking and cellular metabolism in the adult rat prefrontal cortex. Proteomics. 2014;14(12):1574-8. which has been published in final form at DOI: 10.1002/pmic.201300466This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

Published Citation

Farrelly LA, Dicker P, Wynne K, English J, Cagney G, Föcking M, Cotter DR. Adolescent Risperidone treatment alters protein expression associated with protein trafficking and cellular metabolism in the adult rat prefrontal cortex. Proteomics. 2014;14(12):1574-8

Publication Date

01/06/2014

Publisher

Wiley-VCH

PubMed ID

24733778

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