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Circulating miRNAs miR-34a and miR-150 associated with colorectal cancer progression.

journal contribution
posted on 23.11.2019 by Sinéad T. Aherne, Stephen F. Madden, David J. Hughes, Barbara Pardini, Alessio Naccarati, Miroslav Levy, Pavel Vodicka, Paul Neary, Paul Dowling, Martin Clynes

BACKGROUND: Screening for the early detection of colorectal cancer is important to improve patient survival. The aim of this study was to investigate the potential of circulating cell-free miRNAs as biomarkers of CRC, and their efficiency at delineating patients with polyps and benign adenomas from normal and cancer patient groups.

METHODS: The expression of 667 miRNAs was assessed in a discovery set of 48 plasma samples comprising normal, polyp, adenoma, and early and advanced cancer samples. Three miRNAs (miR-34a, miR-150, and miR-923) were further examined in a validation cohort of 97 subjects divided into the same five groups, and in an independent public dataset of 40 CRC samples and paired normal tissues.

RESULTS: High levels of circulating miR-34a and low miR-150 levels distinguished groups of patients with polyps from those with advanced cancer (AUC = 0.904), and low circulating miR-150 levels separated patients with adenomas from those with advanced cancer (AUC = 0.875). In addition, the altered expression of miR-34a and miR-150 in an independent public dataset of forty CRC samples and paired normal tissues was confirmed.

CONCLUSION: We identified two circulating miRNAs capable of distinguishing patient groups with different diseases of the colon from each other, and patients with advanced cancer from benign disease groups.

Funding

Science Foundation Ireland Strategic Research Cluster, Molecular Therapeutics for Cancer Ireland. Grant Agency of the Czech Republic.

History

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The original article is available at www.biomedcentral.com

Published Citation

Aherne ST, Madden SF, Hughes DJ, Pardini B, Naccarati A, Levy M, Vodicka P, Neary P, Dowling P, Clynes M12. Circulating miRNAs miR-34a and miR-150 associated with colorectal cancer progression. BMC Cancer. 2015;15(1):329

Publication Date

30/04/2015

Publisher

Biomed Central

PubMed ID

25924769

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