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Collagen scaffolds functionalised with copper-eluting bioactive glass reduce infection and enhance osteogenesis and angiogenesis both in vitro and in vivo.

journal contribution
posted on 23.11.2019, 11:40 by Emily J. Ryan, Alan J. Ryan, Arlyng Gonzalez Vazquez, Anahí Philippart, Francesca E. Ciraldo, Christopher Hobbs, Valeria Nicolosi, Aldo R. Boccaccini, Cathal Kearney, Fergal J. O'Brien

The bone infection osteomyelitis (typically by Staphylococcus aureus) usually requires a multistep procedure of surgical debridement, long-term systemic high-dose antibiotics, and - for larger defects - bone grafting. This, combined with the alarming rise in antibiotic resistance, necessitates development of alternative approaches. Herein, we describe a one-step treatment for osteomyelitis that combines local, controlled release of non-antibiotic antibacterials with a regenerative collagen-based scaffold. To maximise efficacy, we utilised bioactive glass, an established osteoconductive material with immense capacity for bone repair, as a delivery platform for copper ions (proven antibacterial, angiogenic, and osteogenic properties). Multifunctional collagen-copper-doped bioactive glass scaffolds (CuBG-CS) were fabricated with favourable microarchitectural and mechanical properties (up to 1.9-fold increase in compressive modulus over CS) within the ideal range for bone tissue engineering. Scaffolds demonstrated antibacterial activity against Staphylococcus aureus (up to 66% inhibition) whilst also enhancing osteogenesis (up to 3.6-fold increase in calcium deposition) and angiogenesis in vitro. Most significantly, when assessed in a chick embryo in vivo model, CuBG-CS not only demonstrated biocompatibility, but also a significant angiogenic and osteogenic response, consistent with in vitro studies. Collectively, these results indicate that the CuBG-CS developed here show potential as a one-step osteomyelitis treatment: reducing infection, whilst enhancing bone healing.

Funding

Irish Research Council (GOIPG/2015/3044). RCSI Office of Research and Innovation Seed Fund Award (Grant no. GR 14–0963) Science Foundation Ireland under Grant no. SFI/12/RC/2278 (the AMBER Centre) European Union for a Marie Curie European ReintegrationGrant under H2020 (Project Reference 659715). EU-FP7 Programme, Starting Independent Research Grant (Award No: 238685-CollRegen).

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The original article is available at www.sciencedirect.com

Published Citation

Ryan EJ, Ryan AJ, González-Vázquez A, Philippart A, Ciraldo FE, Hobbs C, Nicolosi V, Boccaccini AR, Kearney CJ, O'Brien FJ. Collagen scaffolds functionalised with copper-eluting bioactive glass reduce infection and enhance osteogenesis and angiogenesis both in vitro and in vivo. Biomaterials. 2019;197:405-416.

Publication Date

01/03/2019

Publisher

Elsevier

PubMed ID

30708184

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