Development of a collagen calcium-phosphate scaffold as a novel b.pdf (2.17 MB)

Development of a collagen calcium-phosphate scaffold as a novel bone graft substitute.

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journal contribution
posted on 22.11.2019, 15:07 by Amir A. Al-Munajjed, John P. Gleeson, Fergal J. O'Brien

Previous investigations have shown that collagen shows excellent biological performance as a scaffold for tissue engineering. As a primary constituent of bone and cartilage, it demonstrates excellent cell adhesion and proliferation. However, in bone tissue engineering, it has insufficient mechanical properties for implantation in a load-bearing defect. The objective of this preliminary study was to investigate the possibility of developing a collagen/calcium-phosphate composite scaffold which would combine the biological performance and the high porosity of a collagen scaffold with the high mechanical stiffness of a calcium-phosphate scaffold. Collagen scaffolds were produced by a lyophilisation process from a collagen slurry. The scaffolds were soaked for different exposure times in solutions of 0.1 M, 0.5 M or 1.0 M NaNH4HPO4 followed by 0.1 M, 0.5 M or 1.0 M CaCl2. Mechanical tests of each scaffold were performed on a uniaxial testing system. Young's moduli were determined from stress-strain curves. The pore structure and porosity of the scaffolds were investigated using micro-computed tomography. A pure collagen scaffold served as a control. All scaffolds showed a significantly increased compressive stiffness relative to the pure collagen scaffolds. The exposure to the 0.5 M solutions showed significantly superior results compared to the other groups. Analysis of the pore structure indicated a decrease in the overall porosity of the composite scaffolds relative to the controls. Regarding mechanical stiffness and porosity, scaffolds after 1 hour exposure to the 0.5 M solutions showed the best properties for bone tissue engineering. Further work will involve producing a scaffold with a more homogeneous calcium phosphate distribution.

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The original article is also available at: http://booksonline.iospress.nl/Content/View.aspx?piid=8536

Published Citation

Al-Munajjed A, Gleeson JP, O'Brien FJ. Development of a collagen calcium-phosphate scaffold as a novel bone graft substitute. Stud Health Technol Inform. 2008; 133:11-20.

Publication Date

01/01/2008

PubMed ID

18376009

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