Eradication of Staphylococcus aureus Biofilm Infections Using Syn.pdf (1.35 MB)

Eradication of Staphylococcus aureus Biofilm Infections Using Synthetic Antimicrobial Peptides.

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posted on 22.11.2019, 15:19 by Marta Zapotoczna, Éanna Forde, Siobhan Hogan, Hilary Humphreys, James P O'Gara, Deirdre Fitzgerald-Hughes, Marc Devocelle, Eoghan O'Neill

Here, we demonstrate that antimicrobial peptides (AMPs) are an effective antibiofilm treatment when applied as catheter lock solutions (CLSs) against S. aureus biofilm infections. The activity of synthetic AMPs (Bac8c, HB43, P18, Omiganan, WMR, Ranalexin, and Polyphemusin) was measured against early and mature biofilms produced by methicillin-resistant S. aureus and methicillin-susceptible S. aureus isolates from patients with device-related infections grown under in vivo-relevant biofilm conditions. The cytotoxic and hemolytic activities of the AMPs against human cells and their immunomodulatory potential in human blood were also characterized. The D-Bac8c2,5Leu variant emerged as the most effective AMP during in vitro studies and was also highly effective in eradicating S. aureus biofilm infection when used in a CLS rat central venous catheter infection model. These data support the potential use of D-Bac8c2,5Leu, alone or in combination with other AMPs, in the treatment of S. aureus intravenous catheter infections.

Funding

This work was supported by grants from the Irish Health Research Board (HRA-POR-2012–52 to E. O’N., H. H. and J. P. O’G.) and the Healthcare Infection Society (to E. O’N.). The authors are grateful to Science Foundation Ireland for supporting the study with an equipment grant (06/RFP/CHO024/EC07).

History

Comments

This is a pre-copyedited, author-produced PDF of an article accepted for publication in Journal of Infectious Diseases following peer review. The version of record Zapotoczna M, Forde É, Hogan S, Humphreys H, O'Gara JP, Fitzgerald-Hughes D, Devocelle M, O'Neill E. Eradication of Staphylococcus aureus Biofilm Infections Using Synthetic Antimicrobial Peptides. Journal of Infectious Diseases. 2017;215(6):975-983. is available online at: https://academic.oup.com/jid/article-lookup/doi/10.1093/infdis/jix062

Published Citation

Zapotoczna M, Forde É, Hogan S, Humphreys H, O'Gara JP, Fitzgerald-Hughes D, Devocelle M, O'Neill E. Eradication of Staphylococcus aureus Biofilm Infections Using Synthetic Antimicrobial Peptides. Journal of Infectious Diseases. 2017;215(6):975-983.

Publication Date

15/03/2017

Publisher

University of Chicago Press

PubMed ID

28453851

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