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Evaluation and optimization of IgY spin column technology in the depletion of abundant proteins from human serum.

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journal contribution
posted on 22.11.2019, 15:11 by Laura Corrigan, Caroline Jefferies, T Clive Lee, Jacqueline Daly

Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin columns. However, modification of the manufacturer's protocol, subjecting samples to two rounds of depletion, improved the number of proteins resolved by 2-DE. With alteration of the manufacturer protocol, the Seppro(®) IgY14 spin column can produce depleted serum with an increased number of spots resolved by 2-DE compared to untreated serum.

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The original article is available at www.wiley.com

Published Citation

Corrigan L, Jefferies C, Clive Lee T, Daly J. Evaluation and optimization of IgY spin column technology in the depletion of abundant proteins from human serum. Proteomics. 2011;11(16):3415-9.

Publication Date

01/08/2011

Publisher

WILEY-VCH Verlag GmbH & Co. KGaA

PubMed ID

21751350

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