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Methicillin resistance and the biofilm phenotype in Staphylococcus aureus.

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journal contribution
posted on 22.11.2019 by Hannah McCarthy, Justine K. Rudkin, Nikki S. Black, Laura Gallagher, Eoghan O'Neill, James P. O'Gara

Antibiotic resistance and biofilm-forming capacity contribute to the success of Staphylococcus aureus as a human pathogen in both healthcare and community settings. These virulence factors do not function independently of each other and the biofilm phenotype expressed by clinical isolates of S. aureus is influenced by acquisition of the methicillin resistance gene mecA. Methicillin-sensitive S. aureus (MSSA) strains commonly produce an icaADBC operon-encoded polysaccharide intercellular adhesin (PIA)-dependent biofilm. In contrast, the release of extracellular DNA (eDNA) and cell surface expression of a number of sortase-anchored proteins, and the major autolysin have been implicated in the biofilm phenotype of methicillin-resistant S. aureus (MRSA) isolates. Expression of high level methicillin resistance in a laboratory MSSA strain resulted in (i) repression of PIA-mediated biofilm production, (ii) down-regulation of the accessory gene regulator (Agr) system, and (iii) attenuation of virulence in murine sepsis and device infection models. Here we review the mechanisms of MSSA and MRSA biofilm production and the relationships between antibiotic resistance, biofilm and virulence gene regulation in S. aureus.

Funding

Research in the author's laboratories is funded by grants from the Irish Health Research Board, the Healthcare Infection Society (UK), and the Irish Research Council.

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This article is also available from http://journal.frontiersin.org/article/10.3389/fcimb.2015.00001/full

Published Citation

McCarthy H, Rudkin JK, Black NS, Gallagher L, O'Neil E, O'Gara JP. Methicillin resistance and the biofilm phenotype in Staphylococcus aureus. Frontiers in Cellular and Infection Microbiology. 2015;5:1.

Publication Date

01/01/2015

PubMed ID

25674541

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