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Optimization Techniques for miRNA Expression in Low Frequency Immune Cell Populations.

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posted on 22.11.2019 by Victoria G Lyons, Natalie L Payne, Claire E McCoy

In this chapter we outline a RNA extraction method for very low immune cell populations isolated from the central nervous system of mice undergoing experimental autoimmune encephalomyelitis. We compare various normalization and quantification techniques to examine miRNA expression. Our data highlight that employing a mean normalization procedure using a number of well-selected housekeeping miRNA genes, followed by absolute quantification with a standard curve generated from a commercial miRNA oligo, gave the most robust and reproducible miRNA expression results.

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This is a post-peer-review, pre-copyedit version of an article published in Inflammation and Cancer. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-4939-7568-6_19

Published Citation

Lyons VG, Payne NL, McCoy CE. Optimization Techniques for miRNA Expression in Low Frequency Immune Cell Populations. Inflammation and Cancer. 2018;1725:237-256.

Publication Date

11/01/2018

Publisher

Humana Press

PubMed ID

29322422

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