Real-time 3D single-molecule localization using experimental poin.pdf (1.85 MB)

Real-time 3D single-molecule localization using experimental point spread functions.

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posted on 22.11.2019, 16:17 by Yiming Li, Markus Mund, Philipp Hoess, Joran Deschamps, Ulf Matti, Bianca Nijmeijer, Vilma Jimenez Sabinina, Jan Ellenberg, Ingmar Schoen, Jonas Ries

We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves minimal uncertainty in 3D on any microscope and is compatible with any PSF engineering approach. We used this method to image cellular structures and attained unprecedented image quality for astigmatic PSFs. The fitter compensates for most optical aberrations and makes accurate 3D super-resolution microscopy broadly accessible, even on standard microscopes without dedicated 3D optics.

Funding

European Research Council (ERC CoG-724489,.), the Deutsche Forschungs Gemeinschaft (DFG RI 2380/2,), by the EMBL Interdisciplinary Postdoc Programme (EIPOD) under Marie Curie Actions COFUND , the 4D Nucleome / 4DN NIH Common Fund (U01 EB021223) and by the European Molecular Biology Laboratory

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The original publication is at www.nature.com

Published Citation

Li Y, Mund M, Hoess P, Deschamps J, Matti U, Nijmeijer B, Sabinina VJ, Ellenberg J, Schoen I, Ries J. Real-time 3D single-molecule localization using experimental point spread functions. Nature Methods. 2018; 15(5):367-369

Publication Date

01/05/2018

Publisher

Nature Publishing Group

PubMed ID

29630062

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