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Reduced pro-inflammatory responses to Staphylococcus aureus bloodstream infection and low prevalence of enterotoxin genes in isolates from patients on haemodialysis.

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posted on 22.11.2019, 15:19 by Sinead McNicholas, Alida Fe Talento, Joanne O'Gorman, Margaret M. Hannan, Maureen Lynch, Catherine M. Greene, Peter J. Conlon, Anna C. Shore, David C. Coleman, Hilary Humphreys, Deirdre Fitzgerald-Hughes

Patients with end-stage renal failure undergo regular haemodialysis (HD) and often develop episodes of Staphylococcus aureus bloodstream infection (BSI), which can re-occur. However, clinically, patients on HD, with S. aureus BSI, respond well to treatment, rarely developing overt signs of sepsis. We investigated the contributions of bacterial virulence and cytokine responses to the clinical course of S. aureus BSI in HD and non-HD patients. Seventy patients were recruited, including 27 (38.6 %) patients on HD. Isolates were spa-typed and virulence and antimicrobial resistance gene carriage was investigated using DNA microarray analysis. Four inflammatory cytokines, IL-6, RANTES, GROγ and leptin, were measured in patient plasma on the day of diagnosis and after 7 days. There was no significant difference in the prevalence of genotypes or antimicrobial resistance genes in S. aureus isolates from HD compared to non-HD patients. The enterotoxin gene cluster (containing staphylococcal enterotoxins seg, sei, sem, sen, seo and seu) was significantly less prevalent among BSI isolates from HD patients compared to non-HD patients. Comparing inflammatory cytokine response to S. aureus BSI in HD patients to non-HD patients, IL-6 and GROγ were significantly lower (p = 0.021 and p = 0.001, respectively) in HD patients compared to other patients on the day of diagnosis and RANTES levels were significantly lower (p = 0.025) in HD patients on day 7 following diagnosis. Lowered cytokine responses in HD patients and a reduced potential for super-antigen production by infecting isolates may partly explain the favourable clinical responses to episodes of S. aureus BSI in HD patients that we noted clinically.

Funding

This work was supported by an Educational Award to SMN from Pfizer Ireland, grant number WS 376235.

History

Comments

The final publication is available at Springer via http://dx.doi.org/10.1007/s10096-016-2767-9

Published Citation

McNicholas S, Fe Talento A, O'Gorman J, Hannan MM, Lynch M, Greene CM, Conlon PJ, Shore AC, Coleman DC, Humphreys H, Fitzgerald-Hughes D. Reduced pro-inflammatory responses to Staphylococcus aureus bloodstream infection and low prevalence of enterotoxin genes in isolates from patients on haemodialysis. European Journal of Clinical Microbiology & Infectious Diseases. 2017;36(1):33-42.

Publication Date

01/01/2017

Publisher

Springer

PubMed ID

27638007

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