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3D-extrusion printing of stable constructs composed of photoresponsive polypeptide hydrogels
journal contribution
posted on 2021-04-16, 15:30 authored by Robert MurphyRobert Murphy, Scott Kimmins, Alan Hibbitts, Andreas HeiseAndreas HeiseThe development of printable hydrogels with functional responsive crosslinking is vital to new age 3D printing materials in biomedical science. Disclosed here is a 3D printable UV responsive crosslinking system based on polypeptides incorporating glutamic acid, isoleucine and nitrobenzyl (NB) protected cysteine groups in a random and block copolymer. The hydrogel ink, encompassing the copolypeptide hydrogel carrier and 4-arm PEG-propiolate, can be readily extruded to produce mechanically stable constructs consisting of a number of geometries due to their remarkable shear-thinning ability. Exploiting the use of a catalyst free thiol-yne click chemistry between the cysteine residues and the propiolate groups upon UV curing, crosslinked hydrogel constructs with greater than 10 layers are fully stabilised and show improved stiffness which allow for them to be easily manipulated. This work can potentially offer a new crosslinking tool to explore in the field of 3D printing, providing highly stable hydrogel structures.
Funding
Science Foundation Ireland (SFI) Principle Investigator Award 13/IA/1840(T)
History
Comments
The original article is available at https://pubs.rsc.orgPublished Citation
Murphy RD, Kimmins S, Hibbitts AJ, Heise A. 3D-extrusion printing of stable constructs composed of photoresponsive polypeptide hydrogels. Polymer Chemistry. 2019;10(34):4675-4682Publication Date
30 July 2019External DOI
Department/Unit
- Amber (Advanced Material & Bioengineering Research) Centre
- Anatomy and Regenerative Medicine
- Chemistry
- Cu00daRAM Centre for Research in Medical Devices
- RCSI Tissue Engineering Group (TERG)
Research Area
- Respiratory Medicine
- Chemistry and Pharmaceutical Sciences
- Biomaterials and Regenerative Medicine
- Immunity, Infection and Inflammation
Publisher
Royal Society of Chemistry (RSC)Version
- Accepted Version (Postprint)