Royal College of Surgeons in Ireland
acsami.2c18837.pdf (2.87 MB)

A biomimetic, bilayered antimicrobial collagen-based scaffold for enhanced healing of complex wound conditions

Download (2.87 MB)
journal contribution
posted on 2023-04-25, 13:24 authored by Matthew McgrathMatthew Mcgrath, Karolina Zimkowska, Katelyn GenoudKatelyn Genoud, Jack Maughan, Javier Gutierrez Gonzalez, Shane BrowneShane Browne, Fergal O'BrienFergal O'Brien

Chronic, nonhealing wounds in the form of diabetic foot ulcers (DFUs) are a major complication for diabetic patients. The inability of a DFU to heal appropriately leads to an open wound with a high risk of infection. Current standards of care fail to fully address either the underlying defective wound repair mechanism or the risk of microbial infection. Thus, it is clear that novel approaches are needed. One such approach is the use of multifunctional biomaterials as platforms to direct and promote wound healing. In this study, a biomimetic, bilayered antimicrobial collagen-based scaffold was developed to deal with the etiology of DFUs. An epidermal, antimicrobial collagen/chitosan film for the prevention of wound infection was combined with a dermal collagen-glycosaminoglycan scaffold, which serves to support angiogenesis in the wound environment and ultimately accelerate wound healing. Biophysical and biological characterization identified an 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide cross-linked bilayered scaffold to have the highest structural stability with similar mechanical properties to products on the market, exhibiting a similar structure to native skin, successfully inhibiting the growth and infiltration of Staphylococcus aureus and supporting the proliferation of epidermal cells on its surface. This bilayered scaffold also demonstrated the ability to support the proliferation of key cell types involved in vascularization, namely, induced pluripotent stem cell derived endothelial cells and supporting stromal cells, with early signs of organization of these cells into vascular structures, showing great promise for the promotion of angiogenesis. Taken together, the results indicate that the bilayered scaffold is an excellent candidate for enhancement of diabetic wound healing by preventing wound infection and supporting angiogenesis.


Science Foundation Ireland through the Advanced Materials and BioEngineering Research (AMBER) Centre (grant SFI/12/RC/2278_2).



The original article is available at

Published Citation

McGrath M, et al. A biomimetic, bilayered antimicrobial collagen-based scaffold for enhanced healing of complex wound conditions ACS Appl Mater Interfaces. 2023;15(14):17444-17458.

Publication Date

31 March 2023

PubMed ID



  • Amber (Advanced Material & Bioengineering Research) Centre
  • Anatomy and Regenerative Medicine
  • Tissue Engineering Research Group (TERG)


American Chemical Society


  • Published Version (Version of Record)