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A standardised Andrographis paniculata Burm. Nees aqueous extract prevents Lipopolysaccharide-induced cognitive deficits through suppression of inflammatory cytokines and oxidative stress mediators

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posted on 30.06.2021, 16:46 by Dahiru Sani, Nasir IO Khatab, Brian KirbyBrian Kirby, Audrey Yong, Shariful Hasan, Hamidon Basri, Johnson Stanslas
Substantial evidence has shown that most cases of memory impairment are associated with increased neuroinflammation and oxidative stress. In this study, the potential of a standardised Andrographis paniculata aqueous extract (APAE) to reverse neuroinflammation and cognitive impairment induced by lipopolysaccharide (LPS) was examined in vivo. Rats were treated with APAE (50, 100, 200, and 400 mg·kg , p.o.) for 7 consecutive days prior to LPS (1 mg·kg , i.p.)-induced neuroinflammation and cognitive impairment. Spatial learning and memory were evaluated using the Morris water maze (MWM) test, while neuroinflammation and oxidative stress were assessed through the measurement of specific mediators, namely, tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-1β superoxide dismutase (SOD), catalase (CAT), antioxidant glutathione (GSH), reactive oxygen species (ROS), and thiobarbituric acid reactive substance (TBARS). Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) were also evaluated. LPS caused significant memory deficits in the 2-day MWM protocol, whereas pretreatment with standardised APAE dose-dependently improved performance in the MWM test. APAE treatment also blocked the LPS-induced hippocampal increase in the concentration and expression of proinflammatory cytokines (TNF-α IL-1β and IL-6) and production of ROS and TBARS and enhanced the activities of AChE and BChE. Furthermore, APAE enhanced the decrease in the levels and expression of hippocampal antioxidant enzymes (SOD and CAT) following LPS-induced neuroinflammation and cognitive deficit. The findings from these studies suggested that standardised APAE improved memory and had potent neuroprotective effects against LPS-induced neurotoxicity. −1 −1

Funding

Malaysia Ministry of Agriculture and Agro-based Industry (NRGS grant, NH612D009)

History

Comments

The original article is available at https://www.sciencedirect.com

Published Citation

Sani D, Khatab NIO, Kirby BP, Yong A, Hasan S, Basri H, Stanslas J. A standardised Andrographis paniculata Burm. Nees aqueous extract prevents Lipopolysaccharide-induced cognitive deficits through suppression of inflammatory cytokines and oxidative stress mediators. J Adv Res. 2018;16:87-97.

Publication Date

30 November 2018

PubMed ID

30899592

Department/Unit

  • School of Pharmacy and Biomolecular Sciences

Research Area

  • Health Professions Education
  • Neurological and Psychiatric Disorders

Publisher

Elsevier

Version

  • Published Version (Version of Record)