Royal College of Surgeons in Ireland
Angiogenin protects motoneurons against hypoxic injury..pdf (661.89 kB)
Download file

Angiogenin protects motoneurons against hypoxic injury.

Download (661.89 kB)
journal contribution
posted on 2022-03-31, 14:15 authored by Jordi Sebastià, Dairin Kieran, Bridget Breen, Matthew A. King, D F. Netteland, Doireann Joyce, Susan F. Fitzpatrick, Cormac T. Taylor, Jochen PrehnJochen Prehn

Cells can adapt to hypoxia through the activation of hypoxia-inducible factor-1 (HIF-1), which in turn regulates the expression of hypoxia-responsive genes. Defects in hypoxic signaling have been suggested to underlie the degeneration of motoneurons in amyotrophic lateral sclerosis (ALS). We have recently identified mutations in the hypoxia-responsive gene, angiogenin (ANG), in ALS patients, and have shown that ANG is constitutively expressed in motoneurons. Here, we show that HIF-1alpha is sufficient and required to activate ANG in cultured motoneurons exposed to hypoxia, although ANG expression does not change in a transgenic ALS mouse model or in sporadic ALS patients. Administration of recombinant ANG or expression of wild-type ANG protected motoneurons against hypoxic injury, whereas gene silencing of ang1 significantly increased hypoxia-induced cell death. The previously reported ALS-associated ANG mutations (Q12L, K17I, R31K, C39W, K40I, I46V) all showed a reduced neuroprotective activity against hypoxic injury. Our data show that ANG plays an important role in endogenous protective pathways of motoneurons exposed to hypoxia, and suggest that loss of function rather than loss of expression of ANG is associated with ALS.



This article is available at

Published Citation

Sebastia J, Kieran D, Breen B, King MA, Netteland DF, Joyce D, Fitzpatrick SF, Taylor CT, Prehn JHM. Angiogenin protects motoneurons against hypoxic injury. Cell Death and Differentiation 2009;16(9):1238-47.

Publication Date


PubMed ID



  • Physiology and Medical Physics