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Associations between host microbiome and inflammation suggest role for host microbiome in driving COVID-19 disease severity.pdf (2.15 MB)

Associations between host microbiome and inflammation suggest role for host microbiome in driving COVID-19 disease severity

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journal contribution
posted on 2024-03-21, 14:27 authored by Rachel MacCann, Tarini Shankar Ghosh, Alejandro Abner Garcia Leon, Junhui Li, Riya Negi, Colette Gaillard, Gurvin Saini, Willard Tinago, Eoin R Feeney, Obada Yousif, Aoife G Cotter, Eoghan De BarraEoghan De Barra, Corinna Sadlier, Peter Doran, Alan Landay, Paul W O'Toole, Patrick W Mallon, All Ireland Infectious Diseases cohort study

Systemic inflammation and innate immune activation are associated with COVID-19 disease severity. Knowledge gaps remain in the relationships between microbiome, inflammation and COVID-19 disease severity. To better characterise these associations, we performed 16SrDNA analysis of stool samples in COVID-19 subjects to explore diversity and taxanomic composition. We correlated these to host inflammatory profiles, derived from soluble plasma biomarkers measured by bead-based fluorescence and electrochemiluminescence immunoassays. Associations of microbial diversity and inflammatory biomarkers on maximal COVID-19 severity (mild, moderate v severe/critical) was explored using logistic regression and weighted gene correlation network analysis (WGCNA). Of 79 subjects, 58% were male and 88% were Caucasian with 36% experiencing mild disease, 22% moderate disease and 40% critical/severe COVID-19. Hierarchical clustering and principal component analysis (PCo) revealed distinct inflammatory clusters that were found to correlate with 4 modules of microbiome profiles. Modules 3 and 4 were associated with both older age and severe/critical disease outcomes. These modules were enriched in pathogenic and inflammatory bacteria that mapped to a pro-inflammatory biomarker cluster. In contrast, module 1 exhibited enrichment of anti-inflammatory bacteria, was associated with younger age and mild/moderate disease outcomes and mapped to a less-inflamed biomarker cluster. This study provides further insights into links between host microbiome, inflammatory responses to SARS-CoV-2 infection and clinical COVID-19 disease severity, suggesting a role for the microbiome in shaping distinct host inflammatory responses to infection. 

Funding

Irish Clinical Academic Training (ICAT) Programme

Wellcome Trust and the Health Research Board (Grant Number 203930/B/16/Z)

Health Service Executive

National Doctors Training and Planning

Health and Social Care, Research and Development Division, Northern Ireland

Science Foundation Ireland (grant numbers 20/COV/0305 and 20/COV/8549)

Philanthropic Donation from Smurfit Kappa

History

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The original article is available at https://www.sciencedirect.com/

Published Citation

MacCann R, et al. Associations between host microbiome and inflammation suggest role for host microbiome in driving COVID-19 disease severity. Microbes Infect. 2023:26(3):105247.

Publication Date

8 November 2023

PubMed ID

37944587

Department/Unit

  • Beaumont Hospital
  • International Health and Tropical Medicine

Publisher

Elsevier B.V.

Version

  • Published Version (Version of Record)

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