Royal College of Surgeons in Ireland
nanomaterials-12-02661-v2.pdf (2.44 MB)

Biomolecular corona stability in association with plasma cholesterol level

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journal contribution
posted on 2023-05-17, 08:47 authored by Duong N. Trinh, Meda Radlinskaite, Jack Cheeseman, Gunter Kuhnle, Helen M. I. Osborn, Paula Meleady, Daniel I. R. Spencer, Marco MonopoliMarco Monopoli
Biomolecular corona is spontaneously formed on the surface of nanoparticles (NPs) when they are in contact with biological fluids. It plays an important role in the colloidal stability of NPs, which is of importance for most of their medical applications and toxicity assessment. While typical studies use either blood plasma or serum from a pooled biobank, it is unclear whether differences in the media, such as cholesterol level or protein concentration, might affect the NP colloidal stability and corona composition. In this study, the silica corona was prepared at particularly low plasma concentrations (3%, v/v–1.98 mg/mL) to identify the critical roles of the protein mass/NP surface ratio and the level of plasma cholesterol on the corona protein pattern and particle stability. While depending on the plasma dilution factor, the corona protein composition could be controlled by keeping the protein/NP constant. The NP colloidal stability was found to strongly correlate with the level of cholesterol in human plasma, particularly due to the high enrichment of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in the corona. A cohort study on plasma samples from individuals with known cholesterol levels was performed to highlight that association, which could be relevant for all corona systems enriched with the LDL.


Irish Research Council and Ludger Ltd (Enterprise Partnership Scheme project EPSPG/2019/511)

MRC and Ludger Ltd. (MR/P015786/1)



The original article is available at

Published Citation

Trinh DN, et al. Biomolecular corona stability in association with plasma cholesterol level. Nanomaterials (Basel). 2022;12(15):2661.

Publication Date

3 August 2022

PubMed ID



  • Chemistry

Research Area

  • Chemistry and Pharmaceutical Sciences
  • Biomaterials and Regenerative Medicine




  • Published Version (Version of Record)