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Dataset of KCNQ1, KCNN4, KATP channel expression and dexamethasone modulation of protein kinase signaling in airway epithelial cells

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journal contribution
posted on 29.07.2022, 11:03 authored by Darina Hynes, Brian HarveyBrian Harvey
Dexamethasone produces anti-secretory responses in airway epithelium through the inhibition of basolateral membrane K+ channels [1–3]. We have used the human bronchial epithelial cell line 16HBE14o− to investigate the effects of dexamethasone on the expression of K+ channels and regulatory protein kinases. The data demonstrate the expression of three distinct K+ channel types – KCNQ1:KCNE3, KCNN4 and KATP which are differentially regulated by protein kinase A and protein kinase C. The data also provide evidence for rapid non-genomic actions of dexamethasone on PKC and PKA phosphorylation and their association with the various K+ channel sub-types. Biotinylation experiments provide data on the effects of dexamethasone on membrane expression of the K+ channels. Antibody co-immunoprecipitation, rtPCR and western blotting data are given for the non-genomic dexamethasone transcription-cell signaling pathway involving Gi-protein coupled receptor, PKC, adenylyl cyclase Type IV, cAMP, PKA and ERK1/2 activation.

Funding

Higher Education Authority of Ireland PRTLI4 grant, National Biophotonics Imaging Platform Ireland

History

Comments

The original article is available at https://www.sciencedirect.com/

Published Citation

Hynes D, Harvey BJ. Dataset of KCNQ1, KCNN4, KATP channel expression and dexamethasone modulation of protein kinase signaling in airway epithelial cells. Data Brief. 2019 ;27:104642

Publication Date

22 October 2019

PubMed ID

31720320

Department/Unit

  • Beaumont Hospital
  • Molecular Medicine

Research Area

  • Respiratory Medicine
  • Endocrinology

Publisher

Elsevier BV

Version

  • Published Version (Version of Record)