Mesenchymal stem cell behavior can be regulated through mechanical signaling, either by dynamic loading or through biomaterial properties. We developed intrinsically responsive tissue engineering scaffolds that can dynamically load cells. Porous collagen- and alginate-based scaffolds were functionalized with iron oxide to produce magnetically active scaffolds. Reversible deformations in response to magnetic stimulation of up to 50% were recorded by tuning the material properties. Cells could attach to these scaffolds and magnetically induced compressive deformation did not adversely affect viability or cause cell release. This platform should have broad application in the mechanical stimulation of cells for tissue engineering applications.
The authors acknowledge support from the TCD MSc program in Bioengineering and Science Foundation Ireland (AMBER, SFI/12/RC/2278). C.J.K. and F.J.O’.B. acknowledge RCSI’s Office of Research and Innovation Summer Research School Program and Seed Fund Award (Grant Number GR 14-0963) and the European Union for a Marie Curie European Reintegration Grant under H2020 (Project Reference 659715). V.N. and C.H. would like to thank the ERC (StG 2DNanocaps) and SFI (PIYRA and AMBER) for their support and the Advanced Microscopy Laboratory, TCD for the provision of their facilities.
CommentsThe original article is available at https://www.cambridge.org/core/journals/mrs-communications/article/development-of-magnetically-active-scaffolds-as-intrinsicallydeformable-bioreactors/6942F02BE94A06A4241EBE7893E3FEF9
Published CitationGilroy DA, Hobbs C, Nicolosi V),Buckley CT, O'Brien FJ, Kearney CJ. Development of magnetically active scaffolds as intrinsically-deformable bioreactors. MRS Communications. Published online: 27 June 2017, pp. 1-8
PublisherCambridge University Press