Royal College of Surgeons in Ireland
Effect of the Rehabilitation Program, ReStOre, on Serum Biomarkers in a Randomized Control Trial of Esophagogastric Cancer Survivors.pdf (2.83 MB)

Effect of the rehabilitation program, ReStOre, on serum biomarkers in a randomized control trial of esophagogastric cancer survivors

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posted on 2022-06-03, 13:03 authored by Susan A Kennedy, Stephanie AnnettStephanie Annett, Margaret R Dunne, Fiona BolandFiona Boland, Linda M O’Neill, Emer M Guinan, Suzanne L Doyle, Emma K Foley, Jessie A Elliott, Conor F Murphy, Annemarie E Bennett, Michelle Carey, Daniel Hillary, Tracy RobsonTracy Robson, John V Reynolds, Juliette Hussey, Jacintha O’Sullivan

Background: The Rehabilitation Strategies Following Esophagogastric cancer (ReStOre) randomized control trial demonstrated a significant improvement in cardiorespiratory fitness of esophagogastric cancer survivors. This follow-up, exploratory study analyzed the biological effect of exercise intervention on levels of 55 serum proteins, encompassing mediators of angiogenesis, inflammation, and vascular injury, from participants on the ReStOre trial.

Methods: Patients >6 months disease free from esophagogastric cancer were randomized to usual care or the 12-week ReStOre program (exercise training, dietary counselling, and multidisciplinary education). Serum was collected at baseline (T0), post-intervention (T1), and at 3-month follow up (T2). Serum biomarkers were quantified by enzyme-linked immunosorbent assay (ELISA).

Results: Thirty-seven patients participated in this study; 17 in the control arm and 20 in the intervention arm. Exercise intervention resulted in significant alterations in the level of expression of serum IP-10 (mean difference (MD): 38.02 (95% CI: 0.69 to 75.35)), IL-27 (MD: 249.48 (95% CI: 22.43 to 476.53)), and the vascular injury biomarkers, ICAM-1 (MD: 1.05 (95% CI: 1.07 to 1.66)), and VCAM-1 (MD: 1.51 (95% CI: 1.04 to 2.14)) at T1. A significant increase in eotaxin-3 (MD: 2.59 (95% CI: 0.23 to 4.96)), IL-15 (MD: 0.27 (95% CI: 0 to 0.54)) and decrease in bFGF (MD: 1.62 (95% CI: -2.99 to 0.26)) expression was observed between control and intervention cohorts at T2 (p<0.05).

Conclusions: Exercise intervention significantly altered the expression of a number of serum biomarkers in disease-free patients who had prior treatment for esophagogastric cancer.

Impact: Exercise rehabilitation causes a significant biological effect on serum biomarkers in esophagogastric cancer survivors.

Clinical trial registration: (NCT03314311).


Science Foundation Ireland 17/TIDA/5053

National Children’s Research Centre and the Children’s Medical & Research Foundation, Crumlin, Ireland, grant code (C/18/9)

Science Foundation Ireland strategic partnership programme, Precision Oncology Ireland, grant code (18/SPP/352)

Health Research Board Ireland (The ReStOre trial, grant number HRA-POR-2014-535)



The original article is available at

Published Citation

Kennedy SA. et al. Effect of the rehabilitation program, ReStOre, on serum biomarkers in a randomized control trial of esophagogastric cancer survivors. Front Oncol. 2021;11:669078

Publication Date

15 September 2021

PubMed ID



  • Data Science Centre
  • School of Pharmacy and Biomolecular Sciences

Research Area

  • Cancer
  • Population Health and Health Services
  • Vascular Biology
  • Immunity, Infection and Inflammation


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  • Published Version (Version of Record)