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Electrochemiluminescent detection of epilepsy biomarker miR-134 using a metal complex light switch

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journal contribution
posted on 03.08.2022, 13:52 authored by Robert J Forster, David HenshallDavid Henshall, Hany El-NaggarHany El-Naggar, Yann Pellegrin, Norman Delanty
The detection of a key biomarker in epilepsy, miR-134, using an environmentally sensitive electrochemiluminescent luminophore, [Ru(DPPZ)2 PIC]2+, is reported, DPPZ is dipyrido[3,2-a:2′,3′-c]phenazine) and PIC is (2,2′-bipyridyl)-2(4-carboxy phenyl) imidazo [4,5][1,10] phenanthroline. A thiolated capture strand is first labelled with [Ru(DPPZ)2 PIC]2+ and then adsorbed onto a gold electrode. No significant electrochemiluminescence, ECL, is observed for immobilised Ru-labelled capture strands which is consistent with the light-switch dye being exposed to the aqueous solution. In sharp contrast, binding of the target turns on ECL. The ECL intensity, IECL, depends on the number of adenine “spacer” bases between the end of the capture sequence and the dye. The ECL intensity for the optimised system increases linearly with increasing miR-134 concentration from 100 nM to approximately 20 μM. Single and double base mismatches produce IECL that are only approximately 30% and 8% respectively of that observed for the fully complementary target reflecting differences in their association constants. Significantly, the presence of BSA protein causes IECL to increase by less 5% in either the single or duplex circumstances. Finally, the ability of the sensor to quantify miR-134 in unprocessed plasma samples from healthy volunteers and people with epilepsy is reported.

Funding

Science Foundation Ireland (SFI) under Grant Number 16/RC/3948

European Regional Development Fund

FutureNeuro industry partners

History

Comments

The original article is available at https://www.sciencedirect.com/

Published Citation

Forster RJ, Henshall DC, El Naggar H, Pellegrin Y, Delanty N. Electrochemiluminescent detection of epilepsy biomarker miR-134 using a metal complex light switch. Bioelectrochemistry. 2022;146:108150.

Publication Date

5 May 2022

PubMed ID

35550256

Department/Unit

  • Beaumont Hospital
  • FutureNeuro Centre
  • Physiology and Medical Physics
  • School of Pharmacy and Biomolecular Sciences

Research Area

  • Neurological and Psychiatric Disorders

Publisher

Elsevier BV

Version

  • Published Version (Version of Record)