Electrochemiluminescent.pdf (1.42 MB)
Electrochemiluminescent detection of epilepsy biomarker miR-134 using a metal complex light switch
journal contribution
posted on 2022-08-03, 13:52 authored by Robert J Forster, David HenshallDavid Henshall, Hany El-NaggarHany El-Naggar, Yann Pellegrin, Norman DelantyNorman DelantyThe detection of a key biomarker in epilepsy, miR-134, using an environmentally sensitive electrochemiluminescent luminophore, [Ru(DPPZ)2 PIC]2+, is reported, DPPZ is dipyrido[3,2-a:2′,3′-c]phenazine) and PIC is (2,2′-bipyridyl)-2(4-carboxy phenyl) imidazo [4,5][1,10] phenanthroline. A thiolated capture strand is first labelled with [Ru(DPPZ)2 PIC]2+ and then adsorbed onto a gold electrode. No significant electrochemiluminescence, ECL, is observed for immobilised Ru-labelled capture strands which is consistent with the light-switch dye being exposed to the aqueous solution. In sharp contrast, binding of the target turns on ECL. The ECL intensity, IECL, depends on the number of adenine “spacer” bases between the end of the capture sequence and the dye. The ECL intensity for the optimised system increases linearly with increasing miR-134 concentration from 100 nM to approximately 20 μM. Single and double base mismatches produce IECL that are only approximately 30% and 8% respectively of that observed for the fully complementary target reflecting differences in their association constants. Significantly, the presence of BSA protein causes IECL to increase by less 5% in either the single or duplex circumstances. Finally, the ability of the sensor to quantify miR-134 in unprocessed plasma samples from healthy volunteers and people with epilepsy is reported.
Funding
Science Foundation Ireland (SFI) under Grant Number 16/RC/3948
European Regional Development Fund
FutureNeuro industry partners
History
Comments
The original article is available at https://www.sciencedirect.com/Published Citation
Forster RJ, Henshall DC, El Naggar H, Pellegrin Y, Delanty N. Electrochemiluminescent detection of epilepsy biomarker miR-134 using a metal complex light switch. Bioelectrochemistry. 2022;146:108150.Publication Date
5 May 2022External DOI
PubMed ID
35550256Department/Unit
- Beaumont Hospital
- FutureNeuro Centre
- Physiology and Medical Physics
- School of Pharmacy and Biomolecular Sciences
Research Area
- Neurological and Psychiatric Disorders
Publisher
Elsevier BVVersion
- Published Version (Version of Record)
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Keywords
EpilepsyPhenanthrolinesMicroRNAsLuminescent MeasurementsBiosensing TechniquesElectrochemical TechniquesCoordination ComplexesBiomarkersElectrochemical biosensorElectrochemiluminescenceEpilepsy biomarkerRuthenium luminophoremiRNAAnalytical ChemistryPhysical Chemistry (incl. Structural)Biochemistry and Cell Biologyenvironmentally sensitiveECLIECLBSACentral Nervous SystemNeurology and Neuromuscular Diseases