Epidemiology and molecular identification of mixed yeast isolates in Malaysia: a way forward
Background and purpose: Invasive candidiasis is one of the most common systemic mycoses, and studies have shown mixed yeast infections. Malaysia lacks mixed yeast culture data.
Materials and methods: Yeast isolates were collected in Sultan Abdul Halim Hospital, North Malaysia, from October 2020 to October 2021. Chromogenic Candida differential agar media and PCR-RFLP were used to identify yeast species.
Results: A total of 206 yeast isolates were collected from different body sites of patients. The majority of the yeast isolates (n=104) were obtained from the urine. Other isolates were extracted from blood (n=52), vaginal swabs (n=45), ear discharge (n=2), tracheal aspirate (n=2), tissue (n=2), skin (n=1), nail (n=1), sputum (n=1), and cerebrospinal fluid (n=1). In total, 200 yeast samples were identified as single species, and six isolates were a mixture of Candida species.
Conclusion: Malaysia lacks accurate epidemiological data on mixed yeast infections. We identified all samples to the species level, including mixed yeast cultures, using the MspI enzyme and PCR-RFLP
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The original article is available at https://publish.kne-publishing.com/Published Citation
Farooq H, Monowar T, V Chinni S, Swe Latt S, Hasliza Zainol N, Shankar Sabesan G. Epidemiology and molecular identification of mixed yeast isolates in Malaysia: a way forward. Curr Med Mycol. 2022;8(3):35-38.Publication Date
20 November 2022External DOI
PubMed ID
37051557Department/Unit
- RCSI + UCD Malaysia Campus (RUMC)
Publisher
Mazandaran University of Medical SciencesVersion
- Published Version (Version of Record)