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Epidemiology and molecular identification of mixed yeast isolates in Malaysia: a way forward

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posted on 2024-06-13, 10:44 authored by Humaira Farooq, Tahmina Monowar, Suresh V Chinni, Swe Swe Latt, Noor Hasliza Zainol, Gokul Shankar Sabesan

Background and purpose: Invasive candidiasis is one of the most common systemic mycoses, and studies have shown mixed yeast infections. Malaysia lacks mixed yeast culture data.

Materials and methods: Yeast isolates were collected in Sultan Abdul Halim Hospital, North Malaysia, from October 2020 to October 2021. Chromogenic Candida differential agar media and PCR-RFLP were used to identify yeast species.

Results: A total of 206 yeast isolates were collected from different body sites of patients. The majority of the yeast isolates (n=104) were obtained from the urine. Other isolates were extracted from blood (n=52), vaginal swabs (n=45), ear discharge (n=2), tracheal aspirate (n=2), tissue (n=2), skin (n=1), nail (n=1), sputum (n=1), and cerebrospinal fluid (n=1). In total, 200 yeast samples were identified as single species, and six isolates were a mixture of Candida species.

Conclusion: Malaysia lacks accurate epidemiological data on mixed yeast infections. We identified all samples to the species level, including mixed yeast cultures, using the MspI enzyme and PCR-RFLP

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The original article is available at https://publish.kne-publishing.com/

Published Citation

Farooq H, Monowar T, V Chinni S, Swe Latt S, Hasliza Zainol N, Shankar Sabesan G. Epidemiology and molecular identification of mixed yeast isolates in Malaysia: a way forward. Curr Med Mycol. 2022;8(3):35-38.

Publication Date

20 November 2022

PubMed ID

37051557

Department/Unit

  • RCSI + UCD Malaysia Campus (RUMC)

Publisher

Mazandaran University of Medical Sciences

Version

  • Published Version (Version of Record)