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Evaluation and optimization of IgY spin column technology in the depletion of abundant proteins from human serum.

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posted on 07.04.2022, 11:10 authored by Laura Corrigan, Caroline Jefferies, Clive LeeClive Lee, Jacqueline DalyJacqueline Daly

Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin columns. However, modification of the manufacturer's protocol, subjecting samples to two rounds of depletion, improved the number of proteins resolved by 2-DE. With alteration of the manufacturer protocol, the Seppro(®) IgY14 spin column can produce depleted serum with an increased number of spots resolved by 2-DE compared to untreated serum.

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Comments

The original article is available at www.wiley.com

Published Citation

Corrigan L, Jefferies C, Clive Lee T, Daly J. Evaluation and optimization of IgY spin column technology in the depletion of abundant proteins from human serum. Proteomics. 2011;11(16):3415-9.

Publication Date

2011-08-01

PubMed ID

21751350

Department/Unit

  • Anatomy and Regenerative Medicine
  • School of Pharmacy and Biomolecular Sciences