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Evaluation of various solvent extracts of Tetrastigma leucostaphylum (Dennst.) alston leaves, a Bangladeshi traditional medicine used for the treatment of diarrhea.

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posted on 05.01.2021, 11:58 by Sajib Rudra, Afroza Tahamina, Nazim U Emon, Md Adnan, Mohammad Shakil, Md Helal Uddin Chowdhury, James Barlow, Mona S Alwahibi, Mohamed Soliman Elshikh, Mohammad Omar Faruque, Shaikh Bokhtear Uddin
Tetrastigma leucostaphylum (TL) is an important ethnic medicine of Bangladesh used to treat diarrhea and dysentery. Hence, current study has been designed to characterize the antidiarrheal (in vivo) and cytotoxic (in vitro) effects of T. leucostaphylum. A crude extract was prepared with methanol (MTL) and further partitioned into n-hexane (NTL), dichloromethane (DTL), and n-butanol (BTL) fractions. Antidiarrheal activity was investigated using castor oil induced diarrhea, enteropooling, and gastrointestinal transit models, while cytotoxicity was evaluated using the brine shrimp lethality bioassay. In antidiarrheal experiments, all doses (100, 200, and 400 mg/kg) of the DTL extract significantly reduced diarrheal stool frequency, volume and weight of intestinal contents, and gastrointestinal motility in mice. Similarly, in the cytotoxicity assay, all extracts exhibited activity, with the DTL extract the most potent (LC50 67.23 μg/mL). GC-MS analysis of the DTL extract identified 10 compounds, which showed good binding affinity toward M3 muscarinic acetylcholine, 5-HT3, Gut inhibitory phosphodiesterase, DNA polymerase III subunit alpha, and UDP-N-acetylglucosamine-1 carboxyvinyltransferase enzyme targets upon molecular docking analysis. Although ADME/T analyses predicted the drug-likeness and likely safety upon consumption of these bioactive compounds, significant toxicity concerns are evident due to the presence of the known phytotoxin, 2,4-di-tert-butylphenol. In summary, T. leucostaphylum showed promising activity, helping to rationalize the ethnomedicinal use and importance of this plant, its safety profile following both acute and chronic exposure warrants further investigation.

Funding

King Saud University, Riyadh, Saudi Arabia, grant number (RSP-2020/173)

History

Comments

The original article is available at https://www.mdpi.com/

Published Citation

Rudra S, Tahamina A, Emon NU, Adnan M, Shakil M, Chowdhury MHU, Barlow JW, Alwahibi MS, Elshikh MS, Faruque MO, Uddin SB. Evaluation of various oslvent extracts of Tetrastigma leucostaphylum (Dennst.) alston leaves, a Bangladeshi traditional medicine used for the treatment of diarrhea. Molecules. 2020;25(21):4994.

Publication Date

28 October 2020

PubMed ID

33126608

Department/Unit

  • Chemistry

Research Area

  • Chemistry and Pharmaceutical Sciences

Publisher

MDPI AG

Version

  • Published Version (Version of Record)

Licence

Exports