Exploiting a neutral BODIPY copolymer as an effective agent for photodynamic antimicrobial inactivation.
journal contributionposted on 2021-12-21, 11:27 authored by Aoibhín A Cullen, Ashwene Rajagopal, Katharina Heintz, Andreas HeiseAndreas Heise, Robert MurphyRobert Murphy, Igor V Sazanovich, Gregory M Greetham, Michael Towrie, Conor Long, Deirdre Fitzgerald HughesDeirdre Fitzgerald Hughes, Mary T Pryce
We report the synthesis and photophysical properties of a neutral BODIPY photosensitizing copolymer (poly-8-(4-hydroxymethylphenyl)-4,4-difluoro-2,6-diethynyl-4-bora- 3a,4a-diaza-s-indacene) containing ethynylbenzene links between the BODIPY units. The copolymer absorbs further towards the red in the UV-vis spectrum compared to the BODIPY precursor. Photolysis of the polymer produces a singlet excited state which crosses to the triplet surface in less than 300 ps. This triplet state was used to form singlet oxygen with a quantum yield of 0.34. The steps leading to population of the triplet state were studied using time-resolved spectroscopic techniques spanning the pico- to nanosecond timescales. The ability of the BODIPY polymer to generate a biocidal species for bactericidal activity in both solution- and coating-based studies was assessed. When the BODIPY copolymer was dropcast onto a surface, 4 log and 6 log reductions in colony forming units/ml representative of Gram-positive and Gramnegative bacteria, respectively, under illumination at 525 nm were observed. The potent broadspectrum antimicrobial activity of a neutral metal-free copolymer when exposed to visible light conditions may have potential clinical applications in infection management.
Towards novel anti-infectives with enhanced wound-healing for diabetic foot infections; CO-releasing star-shaped microbicidal polymers | Funder: Health Research Board / HRB | Grant ID: MRCG-2018-01
Exploiting Singlet Fission: An Innovative Design Strategy For Antimicrobial Materials | Funder: Science Foundation Ireland | Grant ID: Frontiers Programme 2019
Irish Research Council (AAC) (EPSPG/2016/158)
SFI (19/FFP/6882 award)
STFC Central Laser Facility for granting access to the ULTRA system under EU Access Grant (2019 no. 19230040)
CommentsThe original article is available at https://pubs.acs.org/
Published CitationCullen AA. et al. Exploiting a neutral BODIPY copolymer as an effective agent for photodynamic antimicrobial inactivation. J Phys Chem B. 2021;125(6):1550-1557.
Publication Date4 February 2021
- Amber (Advanced Material & Bioengineering Research) Centre
- Beaumont Hospital
- Clinical Microbiology
- CURAM Centre for Research in Medical Devices
- Immunity, Infection and Inflammation
- Chemistry and Pharmaceutical Sciences
- Biomaterials and Regenerative Medicine
PublisherAmerican Chemical Society (ACS)
- Published Version (Version of Record)
Gram-Negative BacteriaGram-Positive BacteriaBoron CompoundsPolymersPhotosensitizing AgentsAnti-Infective AgentsAnti-Bacterial AgentsPhysical SciencesChemical SciencesEngineeringBODIPYsynthesisphotophysical propertiesneutral BODIPYantimicrobial inactivationphotodynamicClinical MicrobiologyMacromolecular and Materials Chemistry not elsewhere classifiedPolymers and PlasticsMedical Bacteriology