Royal College of Surgeons in Ireland
Genetic deletion of microRNA-22 blunts the....pdf (3.79 MB)

Genetic deletion of microRNA-22 blunts the inflammatory transcriptional response to status epilepticus and exacerbates epilepsy in mice

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journal contribution
posted on 2021-08-05, 09:17 authored by Luiz F Almeida Silva, Cristina Ruedell ReschkeCristina Ruedell Reschke, Ngoc Thanh Nguyen, Elena LangaElena Langa, Amaya Sanz RodriguezAmaya Sanz Rodriguez, Rogério Gerbatin, Fernanda R Temp, Mayara L De Freitas, Ronan ConroyRonan Conroy, Gary P Brennan, Tobias EngelTobias Engel, David HenshallDavid Henshall
MicroRNAs perform important roles in the post-transcriptional regulation of gene expression. Sequencing as well as functional studies using antisense oligonucleotides indicate important roles for microRNAs during the development of epilepsy through targeting transcripts involved in neuronal structure, gliosis and inflammation. MicroRNA-22 (miR-22) has been reported to protect against the development of epileptogenic brain networks through suppression of neuroinflammatory signalling. Here, we used mice with a genetic deletion of miR-22 to extend these insights. Mice lacking miR-22 displayed normal behaviour and brain structure and developed similar status epilepticus after intraamygdala kainic acid compared to wildtype animals. Continuous EEG monitoring after status epilepticus revealed, however, an accelerated and exacerbated epilepsy phenotype whereby spontaneous seizures began sooner, occurred more frequently and were of longer duration in miR-22-deficient mice. RNA sequencing analysis of the hippocampus during the period of epileptogenesis revealed a specific suppression of inflammatory signalling in the hippocampus of miR-22-deficient mice. Taken together, these findings indicate a role for miR-22 in establishing early inflammatory responses to status epilepticus. Inflammatory signalling may serve anti-epileptogenic functions and cautions the timing of anti-inflammatory interventions for the treatment of status epilepticus.


CNPq foundation

Science Foundation Ireland awards 13/IA/1891 , 17/CDA/4708, 17/TIDA/5002, 16/RC/3948

European Regional Development Fund

FutureNeuro industry partners

CAPES/PDSE visiting scientist fellowships

Marie S Curie Individual Fellowship (EpimiRGen)

European Union Seventh Framework Programme (FP7/2007–2013) grant agreement n° 602130 (EpimiRNA)



The original article is available at

Published Citation

Almeida Silva LF, et al. Genetic deletion of microRNA-22 blunts the inflammatory transcriptional response to status epilepticus and exacerbates epilepsy in mice. Mol Brain. 2020;13(1):114.

Publication Date

21 August 2020

PubMed ID



  • FutureNeuro Centre
  • Physiology and Medical Physics
  • Public Health and Epidemiology

Research Area

  • Population Health and Health Services
  • Neurological and Psychiatric Disorders
  • Health Professions Education
  • Cancer


BioMed Central


  • Published Version (Version of Record)