Royal College of Surgeons in Ireland
ijms-23-09505-v2.pdf (1.52 MB)

Impact of fluid flow shear stress on osteoblast differentiation and cross-talk with articular chondrocytes

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journal contribution
posted on 2022-11-11, 09:43 authored by Paige Hinton, Katelyn GenoudKatelyn Genoud, James Early, Fergal O'BrienFergal O'Brien, Oran KennedyOran Kennedy
Bone cells, in particular osteoblasts, are capable of communication with each other during bone growth and homeostasis. More recently it has become clear that they also communicate with other cell-types; including chondrocytes in articular cartilage. One way that this process is facilitated is by interstitial fluid movement within the pericellular and extracellular matrices. This stimulus is also an important mechanical signal in skeletal tissues, and is known to generate shear stresses at the micron-scale (known as fluid flow shear stresses (FFSS)). The primary aim of this study was to develop and characterize an in vitro bone–cartilage crosstalk system, to examine the effect of FFSS on these cell types. Specifically, we evaluated the response of osteoblasts and chondrocytes to FFSS and the effect of FFSS-induced soluble factors from the former, on the latter. This system will ultimately be used to help us understand the role of subchondral bone damage in articular cartilage degeneration. We also carried out a comparison of responses between cell lines and primary murine cells in this work. Our findings demonstrate that primary cells produce a more reliable and reproducible response to FFSS. Furthermore we found that at lower magnitudes, direct FFSS produces anabolic responses in both chondrocytes and osteoblasts, whereas higher levels produce more catabolic responses. Finally we show that exposure to osteoblast-derived factors in conditioned media experiments produced similarly catabolic changes in primary chondrocytes.


Science Foundation Ireland, grant number 17/CDA/4699

Advanced Materials and Bioengineering Research (AMBER) Centre (SFI/12/RC/2278 and SFI/12/RC/2278_P2)



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Published Citation

Hinton PV, Genoud KJ, Early JO, O'Brien FJ, Kennedy OD. Impact of fluid flow shear stress on osteoblast differentiation and cross-talk with articular chondrocytes. Int J Mol Sci. 2022;23(16):9505.

Publication Date

22 August 2022

PubMed ID



  • Amber (Advanced Material & Bioengineering Research) Centre
  • Anatomy and Regenerative Medicine
  • Tissue Engineering Research Group (TERG)

Research Area

  • Biomaterials and Regenerative Medicine
  • Chemistry and Pharmaceutical Sciences
  • Immunity, Infection and Inflammation




  • Published Version (Version of Record)