Inhibition of cyclooxygenase -2 and amphiregulin reduces cell proliferation in a colorectal cancer cell line
Background: The role of cyclooxygenase-2 (COX-2) overexpression in colorectal cancers has engaged interest in the role of COX-2 and prostaglandin E2 (PGE2) in cancer cell mitogenesis. Amphiregulin (AR), an epidermal growth factor receptor (EGFR) ligand, has been directly related to PGE2, which activates the AR promoter, causing increased AR expression.
Aims: To identify if COX-2 and AR play a role in human colon cancer cell mitogenesis.
Methods: HCA-7 cells in culture were treated alone or in combination with an amphiregulin neutralising antibody (ARNA) and a selective COX-2 inhibitor (SC236). The mitogenic effects were assessed by enzyme immunoassay to quantify bromodeoxyuridine (BrdU) incorporation.
Results: ARNA (P<0.01) and SC236 (P<0.01) alone both showed a growth inhibitory effect, which was augmented by co-treatment (P<0.001) with both agents. EGFR signalling and COX-2 play roles in colon cancer cell death regulation.
Conclusions: Novel therapeutic strategies should combine elements that target both pathways.
CommentsThe original article is available at http://www.rcsismj.com/ Part of the RCSIsmj collection 2007-8 https://doi.org/10.25419/rcsi.c.6655157.v1
Published CitationMalhotra V, Chopra A. Inhibition of cyclooxygenase -2 and amphiregulin reduces cell proliferation in a colorectal cancer cell line. RCSIsmj. 2008;1(1):18-21
- Undergraduate Research
PublisherRCSI University of Medicine and Health Sciences
- Published Version (Version of Record)