Integrating point-of-care bacterial fluorescence imaging-guided care with continued wound measurement for enhanced wound area reduction monitoring
Aim: This prospective observational study investigated wound area reduction (WAR) outcomes in a complex wound population composed of non-healing acute and chronic wounds. The relationship between bacterial autofluorescence signals and WAR was investigated. Area measurements were collected both manually and digitally, and both methods were compared for accuracy.
Methods: Twenty-six participants with 27 wounds of varying etiologies were observed twice weekly for two weeks. Digital wound measurement, wound bacterial status assessment, and targeted debridement were performed through a point-of-care fluorescence imaging device (MolecuLight® i: X, MolecuLight Inc, Toronto, Canada). The wound area reduction (WAR) rate was calculated using baseline and last visit measurements. Statistical analyses, including t-tests, Fisher exact tests, the Wilcoxon signed rank test for method comparison, and ANOVA for bacterial subgroups, were applied as pertinent.
Results: The overall average WAR was -3.80 cm2, or a decrease of 46.88% (manual measurement), and -2.62 cm2, or a 46.05% decrease (digital measurement via MolecuLight® device). There were no statistically significant differences between the WAR of acute and chronic wounds (p = 0.7877). A stepwise correlation between the WAR and bacterial status classification per fluorescence findings was observed, where persistent bacteria resulted in worse WAR outcomes. An overestimation of wound area by manual measurement was 23% on average.
Conclusion: Fluorescence imaging signals were linked to WAR outcome and could be considered predictive. Wounds exhibiting bacterial loads that persisted at the end of the study period had worse WAR outcomes, while those for which management was able to effectively remove them demonstrated greater WAR. Manual measurement of the wound area consistently overestimated wound size when compared to digital measurement. However, if performed by the same operator, the overestimation was uniform enough that the WAR was calculated to be close to accurate. Notwithstanding, single wound measurements are likely to result in overestimation.
Funding
Irish Research Council, Enterprise Partnership Scheme. Project ID: EPSPG/2016/159
Open access funding provided by IReL
History
Data Availability Statement
The data presented in this study are available on request from the corresponding author. The data are not publicly available due to privacyComments
The original article is available at https://www.mdpi.com/Published Citation
Derwin R, Patton D, Strapp H, Moore Z. Integrating point-of-care bacterial fluorescence imaging-guided care with continued wound measurement for enhanced wound area reduction monitoring. Diagnostics (Basel). 2023;14(1):2.Publication Date
19 December 2023External DOI
PubMed ID
38201311Department/Unit
- School of Nursing and Midwifery
Publisher
MDPIVersion
- Published Version (Version of Record)