Nanofiber topographies enhance platelet-fibrinogen scaffold interactions.pdf (10.21 MB)
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Nanofiber topographies enhance platelet-fibrinogen scaffold interactions.

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journal contribution
posted on 09.06.2022, 16:13 by Martin KennyMartin Kenny, Stephani Stamboroski, Reem Taher, Dorothea Brüggemann, Ingmar SchoenIngmar Schoen

The initial contact with blood and its components, including plasma proteins and platelets, directs the body's response to foreign materials. Natural scaffolds of extracellular matrix or fibrin contain fibrils with nanoscale dimensions, but how platelets specifically respond to the topography and architecture of fibrous materials is still incompletely understood. In this study, we fabricate planar and nanofiber scaffolds from native fibrinogen to characterize the morphology of adherent platelets and activation markers for phosphatidylserine (PS) exposure and α-granule secretion by confocal fluorescence microscopy and scanning electron microscopy (SEM). Different fibrinogen topographies equally support the spreading and granule secretion of washed platelets. In contrast, preincubation of the scaffolds with plasma diminishes platelet spreading on planar fibrinogen surfaces but not on nanofibers. Our data show that the enhanced interactions of platelets with nanofibers results from a higher locally accessible surface area, effectively increasing the ligand density for integrin-mediated responses. Overall, fibrinogen nanofibers direct platelets towards robust adhesion formation and α-granule secretion while minimizing their pro-coagulant activity. Similar results on fibrinogen-coated PDMS substrates with micron-sized 3D features suggest that surface topography could be used more generally to steer blood-materials interactions on different length scales for enhancing the initial wound healing steps.


Emmy Noether program of the German Re-search Foundation (DFG) via grant number 267326782

Fraunhofer TALENTA start

Royal College of Surgeons in Ireland under a StAR lecturership and through the MPharm student-selected projectP scheme




The original article is available at

Published Citation

Kenny M, Stamboroski S, Taher R, Brüggemann D, Schoen I. Nanofiber topographies enhance platelet-fibrinogen scaffold interactions. Adv Healthc Mater. 2022;e2200249

Publication Date

8 May 2022

PubMed ID



  • Irish Centre for Vascular Biology
  • School of Pharmacy and Biomolecular Sciences
  • Undergraduate Research

Research Area

  • Vascular Biology
  • Biomaterials and Regenerative Medicine




  • Published Version (Version of Record)