Nanoparticle biomolecular corona-based enrichment of plasma glycoproteins for N-glycan profiling and application in biomarker discovery
journal contributionposted on 2023-05-16, 16:28 authored by Duong N. Trinh, Richard A. Gardner, Alessandro N. Franciosi, Cormac McCarthy, Michael P. Keane, Mahmoud SolimanMahmoud Soliman, James O'DonnellJames O'Donnell, Paula Meleady, Daniel I. R. Spencer, Marco MonopoliMarco Monopoli
Biomolecular corona formation has emerged as a recurring and important phenomenon in nanomedicine that has been investigated for potential applications in disease diagnosis. In this study, we have combined the "personalized protein corona"with the N-glycosylation profiling that has recently gained considerable interest in human plasma biomarker discovery as a powerful early warning diagnostic and patient stratification tool. We envisioned that the protein corona formation could be exploited as an enrichment step that is critically important in both proteomic and proteoglycomic workflows. By using silica nanoparticles, plasma fibrinogen was enriched to a level in which its proteomic and glycomic "fingerprints"could be traced with confidence. Despite being a more simplified glycan profile compared to full plasma, the corona glycan profile revealed a fibrinogen-derived glycan peak that was found to potentially distinguish lung cancer patients from controls in a pilot study.
Irish Research Council (Enterprise Partnership Scheme Project EPSPG/2019/511)
CommentsThe original article is available at https://pubs.acs.org/
Published CitationTrinh DN, et al. Nanoparticle biomolecular corona-based enrichment of plasma glycoproteins for N-glycan profiling and application in biomarker discovery. ACS Nano. 2022;16(4):5463-5475.
Publication Date28 March 2022
- Irish Centre for Vascular Biology
- School of Pharmacy and Biomolecular Sciences
- Chemistry and Pharmaceutical Sciences
- Biomaterials and Regenerative Medicine
PublisherAmerican Chemical Society (ACS)
- Published Version (Version of Record)
HumansGlycoproteinsPolysaccharidesFibrinogenPilot ProjectsProteomicsNanoparticlesBiomarkersProtein CoronaN-glycosylationbiomarkerglycan profilinglung cancerprotein coronaNanoscience & NanotechnologyBiomolecular coronaBiomarkersMedicinal and Biomolecular Chemistry not elsewhere classifiedCancerProteomics and Intermolecular Interactions (excl. Medical Proteomics)