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PLGA-PEG nanoparticles show minimal risks of interference with platelet function of human platelet-rich plasma

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journal contribution
posted on 12.07.2021, 16:12 by Rana Bakhaidar, Sarah O'NeillSarah O'Neill, Zebunnissa RamtoolaZebunnissa Ramtoola
The expansion of nanotechnology for drug delivery applications has raised questions regarding the safety of nanoparticles (NPs) due to their potential for interacting at molecular and cellular levels. Although polymeric NPs for drug delivery are formulated using FDA-approved polymers such as lactide-and glycolide-based polymers, their interactions with blood constituents, remain to be identified. The aim of this study was to determine the impact of size-selected Poly-lactide-co-glycolide-polyethylene glycol (PLGA-PEG) NPs on platelet activity. The NPs of 113, 321, and 585 nm sizes, were formulated and their effects at concentrations of 0–2.2 mg/mL on the activation and aggregation of platelet-rich plasma (PRP) were investigated. The results showed that NPs of 113 nm did not affect adenosine diphosphate (ADP)-induced platelet aggregation at any NP concentration studied. The NPs of 321 and 585 nm, at concentrations ≥0.25 mg/mL, reduced ADP-activated platelet aggregation. The platelet activation profile remained unchanged in the presence of investigated NPs. Confocal microscopy revealed that NPs were attached to or internalised by platelets in both resting and activated states, with no influence on platelet reactivity. The results indicate minimal risks of interference with platelet function for PLGA-PEG NPs and that these NPs can be explored as nanocarriers for targeted drug delivery to platelets.

Funding

King Abdulaziz University in Jeddah, Saudi Arabia

Ministry of Higher Education, Saudi Arabia

History

Comments

The original article is available at https://www.mdpi.com

Published Citation

Bakhaidar R, O'Neill S, Ramtoola Z. PLGA-PEG nanoparticles show minimal risks of interference with platelet function of human platelet-rich plasma. Int J Mol Sci. 2020;21(24):9716.

Publication Date

19 December 2020

PubMed ID

33352749

Department/Unit

  • School of Pharmacy and Biomolecular Sciences

Research Area

  • Chemistry and Pharmaceutical Sciences
  • Vascular Biology
  • Immunity, Infection and Inflammation
  • Health Professions Education
  • Biomaterials and Regenerative Medicine

Publisher

MDPI AG

Version

  • Published Version (Version of Record)