Pristine graphene induces innate immune training
journal contributionposted on 2021-01-06, 11:47 authored by Filipa Lebre, John B Boland, Pedro Jose Gouveia, Aoife L Gorman, Mimmi LE Lundahl, Roisin I Lynch, Fergal O'BrienFergal O'Brien, Jonathan Coleman, Ed C Lavelle
Graphene-based materials are of increasing interest for their potential use in biomedical applications. However, there is a need to gain a deeper understanding of how graphene modulates biological responses before moving towards clinical application. Innate immune training is a recently described phenomenon whereby cells of the innate immune system are capable of being programmed to generate an increased non-specific response upon subsequent challenge. This has been well established in the case of certain microbes and microbial products. However, little is known about the capacity of particulate materials, such as pristine graphene (pGr), to promote innate immune training. Here we report for the first time that while stimulation with pGr alone does not directly induce cytokine secretion by bone-marrow derived macrophages (BMDMs), it programs them for enhanced secretion of proinflammatory cytokines (IL-6, TNF-α) and a concomitant decrease in production of the regulatory cytokine, IL-10 after Toll-like receptor (TLR) ligand stimulation. This capacity of pGr to program cells for enhanced inflammatory responses could be overcome if the nanomaterial is incorporated in a collagen matrix. Our findings thus demonstrate the potential of graphene to modulate innate immunity over long timescales and have implications for the design and biomedical use of pGr-based materials.
Science Foundation Ireland Investigator Award 12/1A/1421
SFI Research Centre, Advanced Materials and BioEngineering Research (AMBER) under Grant number SFI/12/RC/2278.
CommentsThe original article is available at https://pubs.rsc.org/
Published CitationLebre F , Boland JB , Gouveia P , Gorman AL , Lundahl MLE , Lynch RI , O'Brien FJ , Coleman J , Lavelle EC . Pristine graphene induces innate immune training. Nanoscale. 2020;12(20):11192-11200.
Publication Date6 May 2020
- Anatomy and Regenerative Medicine
- Chemistry and Pharmaceutical Sciences
- Biomaterials and Regenerative Medicine
- Immunity, Infection and Inflammation
PublisherRoyal Society of Chemistry (RSC)
- Accepted Version (Postprint)