Real-time 3D single-molecule localization using experimental point spread functions.
We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves minimal uncertainty in 3D on any microscope and is compatible with any PSF engineering approach. We used this method to image cellular structures and attained unprecedented image quality for astigmatic PSFs. The fitter compensates for most optical aberrations and makes accurate 3D super-resolution microscopy broadly accessible, even on standard microscopes without dedicated 3D optics.
European Research Council (ERC CoG-724489,.), the Deutsche Forschungs Gemeinschaft (DFG RI 2380/2,), by the EMBL Interdisciplinary Postdoc Programme (EIPOD) under Marie Curie Actions COFUND , the 4D Nucleome / 4DN NIH Common Fund (U01 EB021223) and by the European Molecular Biology Laboratory
CommentsThe original publication is at www.nature.com
Published CitationLi Y, Mund M, Hoess P, Deschamps J, Matti U, Nijmeijer B, Sabinina VJ, Ellenberg J, Schoen I, Ries J. Real-time 3D single-molecule localization using experimental point spread functions. Nature Methods. 2018; 15(5):367-369
- School of Pharmacy and Biomolecular Sciences