Synthesis of bilayer films from regenerated cellulose nanofibers and poly(globalide) for skin tissue engineering applications
journal contributionposted on 28.01.2022, 14:44 authored by Heliane R Amaral, James A Wilson, Ronaldo JFC do Amaral, Irina Pasçu, Fernando CS de Oliveira, Cathal KearneyCathal Kearney, Jair CC Freitas, Andreas HeiseAndreas Heise
Commercial cell-based skin regenerative products are highly expensive, carry the risk of rejection and require a long cell culture period to manufacture. This work describes the synthesis of bilayer films from poly(globalide) (PGl) and regenerated cellulose nanofibers (rCNFs) and their use as a cell-free scaffold to support keratinocyte attachment and proliferation. The method is simple, eco-friendly (as the cellulose precursor is obtained from agricultural waste) and of low cost. The rCNFs were produced by acid hydrolysis and PGl was obtained via enzymatic ring-opening polymerization. The bilayer films were synthesized by layer-by-layer casting at ambient temperature. All the films showed a well-defined interface between PGl and cellulose. The produced rCNF/PGl bilayer films showed cell metabolic activity far superior in comparison with pristine PGl regarding the keratinocyte growth, which illustrates the potential use of these materials in skin tissue engineering.
Coordenaç ̃ao de Aperfeiçoa-mento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001(PDSE - 88881.135697/2016-01)
Brazilian agency CNPq (grant 408001/2016-0)
Science Foundation Ireland (SFI), European Regional Development Fund (Grant Number 13/RC/2073)
European Union’s Horizon 2020 research and innovation program (Marie Skłodowska-Curie grant agreement No 713690)
CommentsThe original article is available at hhttps://www.sciencedirect.com/
Published CitationAmaral HR. et al. Synthesis of bilayer films from regenerated cellulose nanofibers and poly(globalide) for skin tissue engineering applications. Carbohydr Polym. 2021;252:117201.
Publication Date13 October 2020
- Amber (Advanced Material & Bioengineering Research) Centre
- Anatomy and Regenerative Medicine
- Tissue Engineering Research Group (TERG)
- Biomaterials and Regenerative Medicine
- Chemistry and Pharmaceutical Sciences
- Accepted Version (Postprint)