Tissue-specific extracellular matrix scaffolds for the regeneration of spatially complex musculoskeletal tissues.pdf (3.76 MB)
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Tissue-specific extracellular matrix scaffolds for the regeneration of spatially complex musculoskeletal tissues

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journal contribution
posted on 26.04.2022, 07:01 authored by Grainne M. Cunniffe, Pedro J. Díaz-Payno, Eamon SheehyEamon Sheehy, Susan E. Critchley, Henrique V. Almeida, Pierluca Pitacco, Simon F. Carroll, Olwyn R. Mahon, Aisling Dunne, Tanya J. Levingstone, Conor J. Moran, Robert Thomas BradyRobert Thomas Brady, Fergal O'BrienFergal O'Brien, Pieter A.J. Brama, Daniel J. Kelly
Biological scaffolds generated from tissue-derived extracellular matrix (ECM) are commonly used clinically for soft tissue regeneration. Such biomaterials can enhance tissue-specific differentiation of adult stem cells, suggesting that structuring different ECMs into multi-layered scaffolds can form the basis of new strategies for regenerating damaged interfacial tissues such as the osteochondral unit. In this study, mass spectrometry is used to demonstrate that growth plate (GP) and articular cartilage (AC) ECMs contain a unique array of regulatory proteins that may be particularly suited to bone and cartilage repair respectively. Applying a novel iterative freeze-drying method, porous bi-phasic scaffolds composed of GP ECM overlaid by AC ECM are fabricated, which are capable of spatially directing stem cell differentiation in vitro, promoting the development of graded tissues transitioning from calcified cartilage to hyaline-like cartilage. Evaluating repair 12-months post-implantation into critically-sized caprine osteochondral defects reveals that these scaffolds promote regeneration in a manner distinct to commercial control-scaffolds. The GP layer supports endochondral bone formation, while the AC layer stimulates the formation of an overlying layer of hyaline cartilage with a collagen fiber architecture better recapitulating the native tissue. These findings support the use of a bi-layered, tissue-specific ECM derived scaffolds for regenerating spatially complex musculoskeletal tissues.

Funding

European Research Council Starter Grant (StemRepair – Project number: 258463)

Science Foundation Ireland (SFI/12/RC/2278; 12/IA/1554)

Enterprise Ireland (CF/2014/4325)

Irish Research Council (GOIPG/2015/3186)

History

Comments

The original article is available at https://www.sciencedirect.com/

Published Citation

Cunniffe GM, et al. Tissue-specific extracellular matrix scaffolds for the regeneration of spatially complex musculoskeletal tissues. Biomaterials. 2018;188:63-73

Publication Date

4 October 2018

PubMed ID

30321864

Department/Unit

  • Amber (Advanced Material & Bioengineering Research) Centre
  • Anatomy and Regenerative Medicine
  • Tissue Engineering Research Group (TERG)

Research Area

  • Chemistry and Pharmaceutical Sciences
  • Biomaterials and Regenerative Medicine
  • Immunity, Infection and Inflammation

Publisher

Elsevier BV

Version

  • Accepted Version (Postprint)